Department of Medical Microbiology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
Medical Faculty, Institute of Virology, Leipzig University, Leipzig, Germany.
J Med Virol. 2020 Dec;92(12):3007-3015. doi: 10.1002/jmv.25765. Epub 2020 Mar 20.
Enteroviruses (EVs) and human parechoviruses (HPeVs) infections are associated with various forms of disease, including gastroenteritis. As information on the molecular epidemiology of these viruses is limited in Ethiopia, the genetic diversity of EV and HPeV was investigated in the Northwestern part of the country. Of the total 450 stool samples obtained from infants and young children with diarrhea, 157 (34.9%) were positive for EV and 49 (10.9%) for HPeV RNA when tested by real-time reverse transcription polymerase chain reaction. Genotyping was performed by sequencing of the EV VP1 gene and the HPeV VP3/VP1 gene, respectively. Genotyping of EV was successful in 118 samples. Thereof, 82 (69.5%) belonged to non-polio EVs as a broad range of genotypes within species C, B, and A. Sabin polioviruses were found in 36 cases. HPeV sequences were also heterogeneous with a relative dominance of genotype 3. In conclusion, diverse EV and HPeV genotypes were found cocirculating in Northwest Ethiopia. The findings highlight the importance of continuous surveillance of these viruses in Ethiopia.
肠道病毒（EVs）和人类细小病毒（HPeVs）感染与各种形式的疾病有关，包括肠胃炎。由于埃塞俄比亚关于这些病毒的分子流行病学信息有限，因此在该国西北部地区对 EV 和 HPeV 的遗传多样性进行了研究。在从腹泻的婴幼儿中获得的总共 450 份粪便样本中，通过实时逆转录聚合酶链反应检测到 157 份（34.9%）EV 和 49 份（10.9%）HPeV RNA 呈阳性。通过对 EV VP1 基因和 HPeV VP3/VP1 基因进行测序分别进行基因分型。在 118 个样本中成功进行了 EV 基因分型。其中，82 个（69.5%）属于非脊髓灰质炎 EV，属于 C、B 和 A 种内的广泛基因型。在 36 例中发现了小儿麻痹症病毒。HPeV 序列也具有异质性，基因型 3 相对占优势。总之，在埃塞俄比亚西北部发现了多种 EV 和 HPeV 基因型的共同流行。这些发现强调了在埃塞俄比亚持续监测这些病毒的重要性。
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