Department of Virology, Centre Pasteur of Cameroon, Yaoundé, Cameroon.
Faculty of Sciences, Department of Microbiology, University of Yaoundé 1, Yaounde, Cameroon.
PLoS One. 2024 May 29;19(5):e0301771. doi: 10.1371/journal.pone.0301771. eCollection 2024.
Human Parechoviruses (HPeVs) have rarely been considered in the virological investigation of Acute Flacid Paralysis (AFP) cases in Africa, where enteric infections are very common. This study investigated the prevalence and genetic diversity of HPeV in 200 children aged ≤ 15 years with AFP in Cameroon from 2018 to 2019. HPeVs were detected in their faecal RNA using 5'-untranslated real-time RT-PCR. Detected HPeVs were typed by phylogenetic comparison with homologous sequences from homotypic reference strains. Overall, HPeV RNA was detected in 11.0% (22/200) of the 200 stool samples tested. Twelve HPeVs were successfully sequenced and reliably assigned to HPeV-A1, A4, A5, A10, A14, A15, A17 and A18 genotypes. Phylogenetic analyses revealed a high genetic variability among the studied HPeVs, as well as between the studied HPeVs and their previously reported counterparts from Cameroon in 2014. These findings suggest that different HPeV genotypes co-circulate in Cameroon without documented epidemics.
人肠道孤儿病毒（HPeVs）在非洲急性弛缓性麻痹（AFP）病例的病毒学调查中很少被考虑，因为在那里肠道感染非常普遍。本研究调查了 2018 年至 2019 年在喀麦隆 200 名年龄≤15 岁的 AFP 儿童中 HPeV 的流行率和遗传多样性。使用 5'-非翻译区实时 RT-PCR 检测粪便 RNA 中的 HPeV。通过与同源参考株的同源序列进行系统发育比较来对检测到的 HPeV 进行分型。总体而言，在 200 份粪便样本中检测到 11.0%（22/200）的 HPeV RNA。成功测序了 12 株 HPeV，并可靠地分配到 HPeV-A1、A4、A5、A10、A14、A15、A17 和 A18 基因型。系统发育分析显示，研究中 HPeV 之间以及研究中 HPeV 与 2014 年喀麦隆之前报道的 HPeV 之间存在高度遗传变异性。这些发现表明，不同的 HPeV 基因型在喀麦隆共同循环，没有记录到流行。
