埃塞俄比亚西北部德布雷塔博尔五岁以下腹泻儿童肠道病毒的分子检测
Molecular Detection of Enteric Viruses in Under-Five Children with Diarrhea in Debre Tabor, Northwest Ethiopia.
作者信息
Gelaw Aschalew, Liebert Uwe G
机构信息
Department of Medical Microbiology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences University of Gondar, Gondar, Ethiopia.
Institute of Virology, Leipzig University, Leipzig, Germany.
出版信息
Infect Drug Resist. 2022 Apr 20;15:1981-1994. doi: 10.2147/IDR.S364142. eCollection 2022.
BACKGROUND
Viral gastroenteritis belongs to the major public health problems of infant and children worldwide. The largest proportion of morbidity and mortality occurs in Sub-Saharan Africa.
PURPOSE
Aimed to assess the burden and genetic diversity of enteric viruses among children with diarrhea.
PATIENTS AND METHODS
A cross-sectional study was undertaken from December 2015 to April 2016 in Debre Tabor. A total of thirty-eight children, who presented with diarrhea at Debre Tabor health centers, were included. Fecal samples were collected and screened for enteric viruses by RT-PCR. Data were analyzed using SPSS software. Descriptive summary techniques were used to display the findings.
RESULTS
Out of thirty-eight children screened, 52.6% were positive for at least one enteric virus. Six (30.0%) of the children had mixed enteric virus infections. Human adenovirus (HAdV) 7 (18.4%) was predominant followed by noroviruses (NoVs) 5 (13.2%), enterovirus (EV) 5 (13.2%), rotavirus A (RVA) 4 (10.5%), human astrovirus (HAstV) 2 (5.3%), and human parechovirus (HPeV) 1 (2.6%). Overall, nineteen different types of enteric virus genotypes were identified. Diverse adenovirus within species A (HAdV-12,-31), B (HAdV-3), C (HAdV-2), and F (HAdV-4) were detected. Norovirus II (GII.4 and GII.6) and norovirus I (GI.2, GI.3, and GI.5) genotypes were found. Sapovirus genotypes within genogroup II (GII.1, GII.5, and GII.6) were identified. Wild-type rotavirus G9 and P[8] genotypes were detected in one of the rotavirus positive samples. Non-polio enteroviruses within species A (coxsackie A virus (CAV) 5, CAV6, and CAV14) and C (enterovirus (EV-C) 99) were also identified. In two of the fecal samples classic HAstV-2 was detected.
CONCLUSION
Diverse enteric viruses were detected in fecal samples from under-five children with diarrhea. The detection of heterogeneous enteric viruses in this small data set highlights the need for extended multicenter studies to describe the burden and genetic diversity of enteric virus.
背景
病毒性肠胃炎是全球婴幼儿主要的公共卫生问题。发病率和死亡率最高的地区是撒哈拉以南非洲。
目的
旨在评估腹泻儿童肠道病毒的负担及基因多样性。
患者与方法
2015年12月至2016年4月在德布雷塔博尔进行了一项横断面研究。纳入了在德布雷塔博尔健康中心出现腹泻症状的38名儿童。收集粪便样本，通过逆转录聚合酶链反应（RT-PCR）筛查肠道病毒。使用SPSS软件分析数据。采用描述性总结技术展示研究结果。
结果
在筛查的38名儿童中，52.6%至少有一种肠道病毒呈阳性。6名（30.0%）儿童有混合肠道病毒感染。人腺病毒（HAdV）7型（18.4%）最为常见，其次是诺如病毒（NoVs）5型（13.2%）、肠道病毒（EV）5型（13.2%）、轮状病毒A（RVA）4型（10.5%）、人星状病毒（HAstV）2型（5.3%）和人细小病毒（HPeV）1型（2.6%）。总体而言，共鉴定出19种不同类型的肠道病毒基因型。检测到A种（HAdV-12、-31）、B种（HAdV-3）、C种（HAdV-2）和F种（HAdV-4）内的多种腺病毒。发现了诺如病毒II型（GII.4和GII.6）以及诺如病毒I型（GI.2、GI.3和GI.5）基因型。鉴定出II基因组（GII.1、GII.5和GII.6）内的萨波病毒基因型。在一份轮状病毒阳性样本中检测到野生型轮状病毒G9和P[8]基因型。还鉴定出A种（柯萨奇A病毒（CAV）5型、CAV6型和CAV14型）和C种（肠道病毒（EV-C）99型）内的非脊髓灰质炎肠道病毒。在两份粪便样本中检测到经典的HAstV-2型。
结论
在5岁以下腹泻儿童的粪便样本中检测到多种肠道病毒。在这个小数据集中检测到的多种肠道病毒凸显了开展扩展的多中心研究以描述肠道病毒负担及基因多样性的必要性。
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