Anunanthini P, Manoj V M, Sarath Padmanabhan T S, Dhivya S, Narayan J Ashwin, Appunu C, Sathishkumar R
Funct Plant Biol. 2019 Jun;46(6):596. doi: 10.1071/FP18189_CO.
The G protein-coupled receptor is one of the major transmembrane proteins in plants. It consists of an α subunit, a β subunit and three γ subunits. Chilling tolerant divergence 1 (COLD1) includes a Golgi pH receptor (GPHR) domain, which maintains cell membrane organisation and dynamics, along with abscisic acid linked G protein-coupled receptor (ABA_GPCR) that regulates the signalling pathways during cold stress. In the present study, we performed characterisation of a homologous COLD1 from the economically important monocot species Oryza sativa L., Zea mays L., Sorghum bicolor (L.)Moench and Erianthus arundinaceus (L.) Beauv. IK 76-81, a wild relative of Saccharum. COLD1 was isolated from E. arundinaceus IK 76-81, analysed for its evolution, domain, membrane topology, followed by prediction of secondary, tertiary structures and functionally validated in all four different monocots. Gene expression studies of COLD1 revealed differential expression under heat, drought, salinity and cold stresses in selected monocots. This is the first study on regulation of native COLD1 during abiotic stress in monocots, which has opened up new leads for trait improvement strategies in this economically important crop species.
G蛋白偶联受体是植物中主要的跨膜蛋白之一。它由一个α亚基、一个β亚基和三个γ亚基组成。耐冷差异蛋白1(COLD1)包含一个高尔基体pH受体(GPHR)结构域,该结构域维持细胞膜的组织和动态,以及脱落酸连接的G蛋白偶联受体(ABA_GPCR),后者在冷胁迫期间调节信号通路。在本研究中,我们对来自经济上重要的单子叶植物水稻、玉米、高粱以及甘蔗野生近缘种斑茅IK 76 - 81中的同源COLD1进行了表征。从斑茅IK 76 - 81中分离出COLD1,分析其进化、结构域、膜拓扑结构,随后预测其二级、三级结构,并在所有四种不同的单子叶植物中进行功能验证。COLD1的基因表达研究揭示了其在所选单子叶植物的热、干旱、盐度和冷胁迫下的差异表达。这是关于单子叶植物非生物胁迫期间天然COLD1调控的首次研究,为这种经济上重要的作物品种的性状改良策略开辟了新的方向。