Peter Swathik Clarancia, Murugan Naveenarani, Mohanan Manoj Vadakkancherry, Sasikumar Sarath Padmanabhan Thelakat, Selvarajan Dharshini, Jayanarayanan Ashwin Narayan, Shivalingamurthy Suresha G, Chennappa Mahadevaiah, Ramanathan Valarmathi, Govindakurup Hemaprabha, Ram Bakshi, Chinnaswamy Appunu
Division of Crop Improvement, ICAR Sugarcane Breeding Institute, Tamil Nadu, Coimbatore, 641007 India.
Division of Crop Production, ICAR Sugarcane Breeding Institute, Tamil Nadu, Coimbatore, 641007 India.
3 Biotech. 2020 Jul;10(7):304. doi: 10.1007/s13205-020-02295-1. Epub 2020 Jun 14.
Plant nuclear factor () is a transcription activating factor, consisting of three subunits, and plays a key regulatory role in many stress-responsive mechanisms including drought and salinity stresses. NF-Ys function both as complex and individual subunits. Considering the importance of sugarcane as a commercial crop with high socio-economic importance and the crop being affected mostly by water deficit stress and salinity stress causing significant yield loss, nuclear transcriptional factor was focused in this study. Plant nuclear factor subunit B2 from (), a wild relative of sugarcane which is known for its drought and salinity stress tolerance, and commercial hybrid Co 86032 () was isolated and characterized Both and genes are 543 bp long that encodes for a polypeptide of 180 amino acid residues. Comparison of and gene sequences revealed nucleotide substitutions at nine positions corresponding to three synonymous and six nonsynonymous amino acid substitutions that resulted in variations in physiochemical properties. However, multiple sequence alignment (MSA) of NF-YB2 proteins showed conservation of functionally important amino acid residues. In silico analysis revealed NF-YB2 to be a hydrophilic and intracellular protein, and EaNF-YB2 is thermally more stable than that of ShNF-YB2. Phylogenetic analysis suggested the lower rate of evolution of . Subcellular localization in sugarcane callus revealed NF-YB2 localization at nucleus that further evidenced it to be a transcription activation factor. Comparative RT-qPCR experiments showed a significantly higher level of expression in when compared to that in the commercial hybrid Co 86032 under drought and salinity stresses. Hence, could be an ideal candidate gene, and its overexpression in sugarcane through genetic engineering approach might enhance tolerance to drought and salinity stresses.
植物核因子（）是一种转录激活因子，由三个亚基组成，在包括干旱和盐胁迫在内的许多胁迫响应机制中发挥关键调节作用。NF-Ys既作为复合体发挥功能，也作为单个亚基发挥功能。鉴于甘蔗作为一种具有高度社会经济重要性的经济作物的重要性，且该作物主要受水分亏缺胁迫和盐胁迫影响，导致产量大幅损失，本研究聚焦于核转录因子。从甘蔗的野生近缘种（）中分离并鉴定了植物核因子亚基B2，该野生近缘种以其耐旱和耐盐胁迫而闻名，同时还对商业杂交种Co 86032（）进行了研究。和基因均长543 bp，编码一个由180个氨基酸残基组成的多肽。和基因序列的比较揭示了九个位置的核苷酸替换，对应三个同义替换和六个非同义替换，导致了理化性质的变化。然而，NF-YB2蛋白的多序列比对（MSA）显示功能重要的氨基酸残基具有保守性。电子分析表明NF-YB2是一种亲水性细胞内蛋白，且EaNF-YB2在热稳定性上比ShNF-YB2更高。系统发育分析表明的进化速率较低。甘蔗愈伤组织中的亚细胞定位显示NF-YB2定位于细胞核，这进一步证明它是一种转录激活因子。比较RT-qPCR实验表明，在干旱和盐胁迫下，与商业杂交种Co 86032相比，中基因的表达水平显著更高。因此，可能是一个理想的候选基因，通过基因工程方法在甘蔗中过表达该基因可能会增强其对干旱和盐胁迫的耐受性。
