Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China; University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, PR China.
Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China; University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, PR China.
Carbohydr Polym. 2020 May 15;236:116048. doi: 10.1016/j.carbpol.2020.116048. Epub 2020 Feb 20.
Pectin is a family of heteropolysaccharides and highly valued for its various bioactivities. Here, a pectin, RP01-1, was purified from roots of Polygala tenuifolia with the molecular weight of 79.1 kDa. Its structure was characterized as alternate 1, 2, 4-linked α-Rhap and 1, 4-linked α-GalpA constituted the backbone, with branches of terminal (T) -, 1, 4- and 1, 3, 6-linked β-Galp, T-, 1, 5- and 1, 3, 5-linked α-Araf substituted at C-4 of 1, 2, 4-linked α-Rhap. The fluorescence spectroscopic analysis indicated that RP01-1 induced neurite outgrowth in PC12 cells and primary cortex neurons in dose dependent manner with no significant cytotoxicity. To disclose the mechanism of this phenomenon, we found that RP01-1 might induce neuritogenesis in PC12 cells via increasing BDNF expression, and promoting the phosphorylation of AKT, ERK, CREB. The results suggest that AKT, ERK, CREB related signaling pathways might implicate in neuritogenesis-inducing activity of RP01-1.
果胶是一类杂多糖，因其具有多种生物活性而备受重视。在这里，从远志根中纯化得到一种果胶 RP01-1，其分子量为 79.1 kDa。其结构特征为交替的 1,2,4-连接的α-Rhap 和 1,4-连接的α-GalpA 构成主链，支链为末端 (T)-、1,4-和 1,3,6-连接的β-Galp、T-、1,5-和 1,3,5-连接的α-Araf，取代 1,2,4-连接的α-Rhap 的 C-4。荧光光谱分析表明，RP01-1 以剂量依赖的方式诱导 PC12 细胞和原代皮质神经元的神经突生长，而没有明显的细胞毒性。为了揭示这一现象的机制，我们发现 RP01-1 可能通过增加 BDNF 表达，促进 AKT、ERK、CREB 的磷酸化，从而诱导 PC12 细胞的神经突发生。结果表明，AKT、ERK、CREB 相关信号通路可能参与了 RP01-1 诱导神经突发生的活性。
