College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; Fishery Resources and Environmental Science Experimental Station of The Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
Fishery Resources and Environmental Science Experimental Station of The Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
Toxicol Appl Pharmacol. 2020 May 1;394:114957. doi: 10.1016/j.taap.2020.114957. Epub 2020 Mar 12.
The adverse effects of triphenyltin (TPT) on aquatic systems have attracted much attention because TPT is widely used and prevalent in aquatic environments. Here, zebrafish embryos/larvae were exposed to TPT (0, 0.039, 0.39, and 3.9 nM; 0, 15, 150 and 1500 ng/L) for 7 or 14 days to determine its toxic effects on the hypothalamic-pituitary-thyroid (HPT) axis. The results showed that whole-body total T4 and T3 levels were significantly decreased, which was accompanied by the significant upregulation of the expression of the dio1, dio2 and ugt1ab genes after exposure to TPT for 7 and 14 days. Genes related to thyroid hormone synthesis (crh, tshβ, nis, tpo and tg) were upregulated at both 7 and 14 days after TPT exposure. This might have been due to the positive feedback regulation of the HPT axis, which is caused by a decrease in thyroid hormone in the whole body in zebrafish. In addition, the survival rates and body lengths were reduced after treatment with TPT for 7 and 14 days. This indicated that TPT caused adverse effect on the development of zebrafish embryos/larvae. In summary, the results suggested that TPT caused thyroid disruption and developmental toxicity in zebrafish larvae.
三苯基锡（TPT）对水生系统的不良影响引起了广泛关注，因为 TPT 在水生环境中被广泛使用和普遍存在。在这里，我们将斑马鱼胚胎/幼鱼暴露于 TPT（0、0.039、0.39 和 3.9 nM；0、15、150 和 1500 ng/L）中 7 或 14 天，以确定其对下丘脑-垂体-甲状腺（HPT）轴的毒性作用。结果表明，暴露于 TPT 7 和 14 天后，全身总 T4 和 T3 水平显著降低，同时 dio1、dio2 和 ugt1ab 基因的表达显著上调。甲状腺激素合成相关基因（crh、tshβ、nis、tpo 和 tg）在 TPT 暴露 7 天和 14 天后均上调。这可能是由于全身甲状腺激素减少引起的 HPT 轴的正反馈调节。此外，暴露于 TPT 7 和 14 天后，生存率和体长降低。这表明 TPT 对斑马鱼胚胎/幼鱼的发育产生了不良影响。综上所述，研究结果表明，TPT 导致了斑马鱼幼鱼的甲状腺功能紊乱和发育毒性。
