Wang Bingke, Zhang Chunnuan, Ma Jianshuang, Wang Yanhui, Zhang Ling, Yang Xingli, Jia Tao, Zhang Kaisong, Zhang Qin
Henan Academy of Fishery Sciences, Zhengzhou 450044, China.
Henan Fishery Engineering Technology Research Center, Zhengzhou 450044, China.
Antioxidants (Basel). 2024 Sep 26;13(10):1173. doi: 10.3390/antiox13101173.
This experiment mainly explored the protective role of sulforaphane (SFN) against physiological toxicity of triphenyltin (TPT) in . In total, 320 Fish (56.90 ± 0.40 g) were randomly divided into four groups with four replicates each. The control group was fed the basal diet, the TPT group (TPT) was exposed to 10 ng L TPT on the basis of the control group, the SFN + TPT group (TPT + SFN) was fed a diet supplemented with 10 mg kg SFN on the TPT group, and the SFN group (SFN) was fed a diet supplemented with 10 mg kg SFN. After 56 days of breeding trials, the results showed that TPT exposure resulted in a remarkable decrease ( < 0.05) in final weight, weight gain rate (WGR), specific growth rate (SGR), and condition factor (CF), but an increase ( < 0.05) in feed conversion ratio (FCR) and hepatosomatic index (HSI) of fish. TPT treatment decreased ( < 0.05) the amounts of hematocrit (Hct) and hemoglobin (Hb), plasma complement component 3 (C3) and C4 contents, alternative complement pathway (ACH50), acid phosphatase (ACP) and lysozyme (LZM) activities, liver glutathione (GSH) content, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX) activities, interleukin 10 (), and mRNA expressions, but increased ( < 0.05) plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, liver malonaldehyde (MDA) content, tumor Cyclooxygenase 2 (), and necrosis factor α (), , and mRNA expressions. A histological analysis of the liver showed that a higher occurrence rates of the hepatocyte hypertrophy, nuclear disappearance and hepatocyte vacuolization was observed in the hepatocytes of fish exposed to TPT, and it was accompanied by the dilation of hepatic sinusoids. In addition, the toxicity induced by TPT was significantly improved in the groups that were treated with SFN, and SFN was able to improve growth performance and immunity, alleviate TPT-induced changes in inflammatory factors, ameliorate oxidative stress, and increase the activity of antioxidant enzymes ( < 0.05). The addition of SFN also alleviated liver damage caused by TPT and protected the structural integrity of the liver. Overall, these findings suggest that TPT inhibited the growth, immunity, and antioxidant capacity of Dietary SFN could be beneficial for growth promotion, immunity, antioxidant capacity, and protection of liver structural integrity. Therefore, SFN is a prospective feed supplement for ameliorating the damage caused to fish by TPT contamination.
本实验主要探究了萝卜硫素（SFN）对三苯基锡（TPT）在[具体对象未提及]中的生理毒性的保护作用。总共320条鱼（56.90±0.40克）被随机分为四组，每组四个重复。对照组投喂基础饲料，TPT组在对照组基础上暴露于10纳克/升的TPT，SFN + TPT组在TPT组基础上投喂添加10毫克/千克SFN的饲料，SFN组投喂添加10毫克/千克SFN的饲料。经过56天的养殖试验，结果表明，暴露于TPT导致鱼的终末体重、增重率（WGR）、特定生长率（SGR）和肥满度（CF）显著降低（P<0.05），但饲料转化率（FCR）和肝体指数（HSI）增加（P<0.05）。TPT处理降低了（P<0.05）血细胞比容（Hct）和血红蛋白（Hb）含量、血浆补体成分3（C3）和C4含量、替代补体途径（ACH50）、酸性磷酸酶（ACP）和溶菌酶（LZM）活性、肝脏谷胱甘肽（GSH）含量、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX）活性、白细胞介素10（IL-10）以及相关mRNA表达，但增加了（P<0.05）血浆丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）活性、肝脏丙二醛（MDA）含量、肿瘤环氧化酶2（COX-2）、肿瘤坏死因子α（TNF-α）以及相关mRNA表达。肝脏组织学分析表明，暴露于TPT的鱼肝细胞中肝细胞肥大、核消失和肝细胞空泡化的发生率较高，并伴有肝血窦扩张。此外，在使用SFN处理的组中，TPT诱导的毒性得到显著改善，SFN能够提高生长性能和免疫力，减轻TPT诱导的炎症因子变化，改善氧化应激，并增加抗氧化酶活性（P<0.05）。添加SFN还减轻了TPT引起的肝脏损伤，保护了肝脏的结构完整性。总体而言，这些发现表明TPT抑制了[具体对象未提及]的生长、免疫和抗氧化能力。日粮中的SFN有利于促进生长、提高免疫力、增强抗氧化能力以及保护肝脏结构完整性。因此，SFN是一种有望减轻TPT污染对鱼类造成损害的饲料添加剂。
