National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China.
Department of Parasitology, Harbin Medical University, 194 Xuefu Road, Harbin, 150081, China.
Immunol Lett. 2020 Jun;222:29-39. doi: 10.1016/j.imlet.2020.03.002. Epub 2020 Mar 12.
Although many vaccines have been designed to induce effective mucosal immune responses against HIV-1, designing an effective HIV-1 vaccine remains a challenge. Bacterium-like particles (BLPs) are a new type of vector used to induce mucosal immune responses, and have already been used for some vaccines against respiratory tract viruses. In this study, we designed a mucosal vaccine against HIV-1 based on BLPs. The vaccine was used to immunize both mice and guinea pigs via intramuscular (i.m.) injection or intranasal (i.n.) drip. We found that gp120 trimers bound to BLPs delivered via i.n. drip successfully induced Env-specific secretory IgA (sIgA) at mucosal sites in mice. Furthermore, nasal washes from guinea pigs immunized via i.n. drip showed neutralizing activity against HIV-1 tier 1 pseudoviruses. Thus, gp120 trimers bound to BLPs may be an effective vaccine strategy against HIV-1.
尽管已经设计出许多疫苗来诱导针对 HIV-1 的有效黏膜免疫反应，但设计出有效的 HIV-1 疫苗仍然是一个挑战。细菌样颗粒（BLPs）是一种新型载体，用于诱导黏膜免疫反应，已经用于一些针对呼吸道病毒的疫苗。在这项研究中，我们设计了一种基于 BLPs 的针对 HIV-1 的黏膜疫苗。该疫苗通过肌肉内（i.m.）注射或鼻内（i.n.）滴注的方式用于免疫小鼠和豚鼠。我们发现，通过 i.n.滴注递送的与 BLPs 结合的 gp120 三聚体成功地在小鼠的黏膜部位诱导了 Env 特异性分泌型 IgA（sIgA）。此外，通过 i.n.滴注免疫的豚鼠的鼻洗液显示出针对 HIV-1 1 级假病毒的中和活性。因此，与 BLPs 结合的 gp120 三聚体可能是一种针对 HIV-1 的有效疫苗策略。
