Department of Oral & Craniofacial Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
Department of Oral & Craniofacial Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia; Department of Oral Biology and Biomedical Sciences, Faculty of Dentistry, MAHSA University, Selangor, Malaysia.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2020 May;129(5):484-492. doi: 10.1016/j.oooo.2020.01.009. Epub 2020 Mar 13.
The aim of this study was to evaluate the chemopreventive activity of Malaysian jungle Tualang honey (TH) after oral carcinogenesis induced with 4-nitroquinoline 1-oxide (4 NQO).
A total of 28 male Sprague-Dawley (SD) rats were distributed into 4 groups as follows: group 1 (nontreated group); group 2 (control), which received 4 NQO during 8 weeks in drinking water only; and groups 3 and 4, which received 4 NQO for 8 weeks in drinking water and treated with TH 1000 mg/kg and 2000 mg/kg by oral gavage for 10 weeks. All rats from all experiments were sacrificed after 22 weeks, and the incidence of oral neoplasms and histopathologic changes were microscopically evaluated. Moreover, immunohistochemical expression was analyzed in tongue specimens by using image analysis software. The expression of particular genes associated with oral cancer were assessed by using RT Profiler PCR Array (Qiagen, Germantown, MD).
TH significantly reduced the incidence of oral squamous cell carcinoma (OSCC) and suppressed cancer cell proliferation via diminishing the expression of CCND1, EGFR, and COX-2. Furthermore, TH preserved cellular adhesion (epithelial polarity) through overexpression of β-catenin and e-cadherin and inhibited the OSCC aggressiveness by downregulating TWIST1 and RAC1.
Our data suggest that TH exerts chemopreventive activity in an animal model in which oral cancer was induced by using 4 NQO.
本研究旨在评估马来西亚丛林塔鲁盎蜂蜜（TH）在经 4-硝基喹啉 1-氧化物（4NQO）诱导口腔癌变后的化学预防活性。
总共 28 只雄性 Sprague-Dawley（SD）大鼠分为 4 组：第 1 组（未治疗组）；第 2 组（对照组），仅在饮用水中接受 4NQO8 周；第 3 组和第 4 组，在饮用水中接受 4NQO8 周，通过口服灌胃接受 TH1000mg/kg 和 2000mg/kg10 周。所有实验大鼠均在 22 周后处死，通过显微镜评估口腔肿瘤的发生率和组织病理学变化。此外，还通过图像分析软件分析舌标本中的免疫组织化学表达。通过使用 RT Profiler PCR 阵列（Qiagen，Germantown，MD）评估与口腔癌相关的特定基因的表达。
TH 显著降低了口腔鳞状细胞癌（OSCC）的发生率，并通过降低 CCND1、EGFR 和 COX-2 的表达来抑制癌细胞增殖。此外，TH 通过过表达β-连环蛋白和 E-钙黏蛋白来维持细胞黏附（上皮极性），并通过下调 TWIST1 和 RAC1 来抑制 OSCC 的侵袭性。
我们的数据表明，TH 在经 4NQO 诱导的动物模型中表现出化学预防活性。
