Department of Ecology and Biology, University of Tuscia, Via S. Camillo de Lellis 44, 01100 Viterbo, VT, Italy; ISPRA, Italian National Institute for Environmental Protection and Research, Via di Castel Romano 100, 00144 Roma, RM, Italy.
ISPRA, Italian National Institute for Environmental Protection and Research, Via di Castel Romano 100, 00144 Roma, RM, Italy.
Mar Pollut Bull. 2020 May;154:111050. doi: 10.1016/j.marpolbul.2020.111050. Epub 2020 Mar 12.
Microplastic presence in the marine environment has generated considerable concern. Many procedures for microplastics detection in fish gastrointestinal tract have been recently developed. In this study, we compared efficiencies of two common procedures applied for the digestion of organic matter (10% KOH; 15% HO) with a new proposal (mixture of 5% HNO and 15% HO). We considered ecological diversity among species and differences in their diet compositions as factors that could affect the efficiency and feasibility of analytical approaches. Our aim was to understand whether either one of the three protocols might be suitable for all species or it might be more advisable to select a method according to the gut content determined by different food preferences. The results showed that the trophic level and feeding habits should be considered for protocol selection. Finally, we applied the best protocols on samples from the Tyrrhenian sea.
微塑料在海洋环境中的存在引起了相当大的关注。最近已经开发出许多用于检测鱼类胃肠道中微塑料的方法。在这项研究中，我们比较了两种常用于消化有机物的常用方法（10%KOH；15%HO）和一种新方法（5%HNO 和 15%HO 的混合物）的效率。我们考虑了物种间的生态多样性以及它们的饮食组成差异作为可能影响分析方法效率和可行性的因素。我们的目的是了解这三种方案中的任何一种是否可能适用于所有物种，或者根据不同的食物偏好确定的肠道内容物选择一种方法是否更为明智。结果表明，应根据营养水平和饮食习惯选择方案。最后，我们将最佳方案应用于来自第勒尼安海的样本。
