Department of Infection and Immunology, Konkuk University School of Medicine, Seoul, Korea.
BK21 plus, Department of Cellular and Molecular Medicine, Konkuk University School of Medicine, Seoul, Korea.
Int J Med Sci. 2020 Feb 4;17(4):428-436. doi: 10.7150/ijms.41899. eCollection 2020.
: This study was designed to assess the effect of repetitive exposure to intravenous anesthetic agents on the immunity in mice. : The mice were divided into six groups: three intravenous anesthetic agents groups (dexmedetomidine, midazolam and propofol groups), and three corresponding control groups (C, C, and C groups). The intravenous injections were administered once per day for 5 days. The immunity of mice was checked after the last intravenous injection. Histopathology and immunochemistry of liver and kidneys were evaluated. Cytokine levels in the blood was also checked. vs. evaluated with cytokine levels in the blood. : Cluster of differentiation (CD)4 T cells were significantly less expressed in dexmedetomidine and propofol groups, compared with the corresponding control groups [34.08 ± 5.63% in the dexmedetomidine group vs. 59.74 ± 8.64% in the C group, ; 25.28 ± 7.28% in the propofol group vs. 61.12 ± 2.70% in the C group, ]. Apoptosis of CD4 T cells was increased significantly in dexmedetomidine and propofol groups, compared with the corresponding control groups. Histopathological findings of liver and kidneys did not show any specific differences of any of three intravenous anesthetic agents groups with their corresponding control groups, although immunohistochemical examination indicated significantly lower expression of Toll-like receptor-4 from liver and kidneys in dexmedetomidine and propofol groups. The cytokine levels were not different between the groups. : Repetitive exposure to dexmedetomidine and propofol reduced the expression of CD4 T cells but did not induce any significant liver or kidney injuries.
本研究旨在评估重复静脉麻醉剂暴露对小鼠免疫的影响。
三组静脉麻醉剂组（右美托咪定、咪达唑仑和丙泊酚组）和三组相应的对照组（C、C 和 C 组）。每天进行一次静脉注射，共 5 天。最后一次静脉注射后检查小鼠的免疫功能。评估肝脏和肾脏的组织病理学和免疫化学。还检查了血液中的细胞因子水平。
与血液中的细胞因子水平相比，右美托咪定和丙泊酚组的 CD4 T 细胞表达明显减少[右美托咪定组为 34.08 ± 5.63%，C 组为 59.74 ± 8.64%；丙泊酚组为 25.28 ± 7.28%，C 组为 61.12 ± 2.70%]。与相应的对照组相比，右美托咪定和丙泊酚组的 CD4 T 细胞凋亡明显增加。三组静脉麻醉剂组与相应对照组的肝脏和肾脏的组织病理学检查均未见明显差异，尽管免疫组化检查表明右美托咪定和丙泊酚组肝脏和肾脏 Toll 样受体-4 的表达明显降低。细胞因子水平在各组之间无差异。
重复暴露于右美托咪定和丙泊酚可降低 CD4 T 细胞的表达，但不会引起明显的肝或肾损伤。
