Center for Research and Technology of Precision Medicine, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, Guangdong, China.
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, Guangdong, China.
Int J Biol Sci. 2020 Feb 10;16(7):1180-1193. doi: 10.7150/ijbs.41148. eCollection 2020.
This study focused on investigating the relationships of TAF1L expression and clinical features or pathological stages of oral squamous cell carcinoma (OSCC), and its potential roles of TAF1L on OSCC development. Western blot and immunohistochemical staining were used to detect TAF1L expression in OSCC tissues and cells. Effects of TAF1L on OSCC cells were examined by cell proliferation assay, wound healing assay, transwell chamber assay, flow cytometry analysis and siRNA technique. Cellular key proteins related to cell autophagy and apoptosis were evaluated by Western blot and immunofluorescent staining. Moreover, functions of TAF1L on OSCC process were observed in nude mouse model. Testing results showed that expression of TAF1L protein was higher in OSCC tissues than that in normal oral epithelial or paracancerous tissues. Additionally, the level of TAF1L protein expression was upregulated in OSCC cell lines, compared to that in normal oral epithelial cells. Furthermore, cell proliferation, migration, autophagy and apoptosis were modulated post siRNA- treatment . Especially, TAF1L knockdown-induced apoptotic activation on OSCC cells could be rescued by autophagic activator (Rapamycin). Moreover, that overexpression of TAF1L protein could promote the growth of OSCC cell xenografts was confirmed in nude mouse model. Taken together, it suggests that TAF1L may facilitate OSCC cells to escape cell apoptosis via autophagic activation for enhancing OSCC development.
本研究旨在探讨 TAF1L 表达与口腔鳞状细胞癌（OSCC）临床特征和病理分期的关系，以及 TAF1L 对 OSCC 发生发展的潜在作用。采用 Western blot 和免疫组织化学染色检测 OSCC 组织和细胞中 TAF1L 的表达。通过细胞增殖试验、划痕愈合试验、Transwell 室试验、流式细胞术分析和 siRNA 技术检测 TAF1L 对 OSCC 细胞的影响。通过 Western blot 和免疫荧光染色评估与细胞自噬和凋亡相关的细胞关键蛋白。此外，在裸鼠模型中观察 TAF1L 对 OSCC 过程的作用。实验结果表明，TAF1L 蛋白在 OSCC 组织中的表达高于正常口腔上皮或癌旁组织。此外，与正常口腔上皮细胞相比，TAF1L 蛋白表达水平在 OSCC 细胞系中上调。进一步的研究表明，siRNA 处理后可调节细胞增殖、迁移、自噬和凋亡。特别是，自噬激活剂（雷帕霉素）可挽救 TAF1L 敲低诱导的 OSCC 细胞凋亡激活。此外，在裸鼠模型中证实，TAF1L 蛋白过表达可促进 OSCC 细胞异种移植物的生长。综上所述，这表明 TAF1L 可能通过自噬激活促进 OSCC 细胞逃避细胞凋亡，从而增强 OSCC 的发展。
