Lavoie Andréanne, Liu Bao-Hua
Department of Biology, University of Toronto Mississauga, Mississauga, ON, Canada.
Department of Cell and Systems Biology, University of Toronto, Toronto, ON, Canada.
Front Mol Neurosci. 2020 Feb 27;13:9. doi: 10.3389/fnmol.2020.00009. eCollection 2020.
Canine adenovirus-2 (CAV) is a canine pathogen that has been used in a variety of applications, from vaccines against more infectious strains of CAV to treatments for neurological disorders. With recent engineering, CAV has become a natural choice for neuroscientists dissecting the connectivity and function of brain circuits. Specifically, as a reliable genetic vector with minimal immunogenic and cytotoxic reactivity, CAV has been used for the retrograde transduction of various types of projection neurons. Consequently, CAV is particularly useful when studying the anatomy and functions of long-range projections. Moreover, combining CAV with conditional expression and transsynaptic tracing results in the ability to study circuits with cell- and/or projection-type specificity. Lastly, with the well-documented knowledge of viral transduction, new innovations have been developed to increase the transduction efficiency of CAV and circumvent its tropism, expanding the potential of CAV for circuit analysis.
犬腺病毒2型（CAV）是一种犬类病原体，已被用于多种应用，从针对更具传染性的CAV毒株的疫苗到神经疾病的治疗。随着最近的工程改造，CAV已成为神经科学家剖析脑回路连接性和功能的自然选择。具体而言，作为一种具有最小免疫原性和细胞毒性反应的可靠基因载体，CAV已被用于各种类型投射神经元的逆行转导。因此，在研究长距离投射的解剖结构和功能时，CAV特别有用。此外，将CAV与条件表达和跨突触追踪相结合，能够以细胞和/或投射类型特异性研究回路。最后，基于对病毒转导的充分了解，人们开发了新的创新方法来提高CAV的转导效率并规避其嗜性，从而扩大了CAV在回路分析中的潜力。
