评估Myc基因表达作为提高不孕女性着床成功率的预防标志物。
Evaluation of Myc Gene Expression as a Preventive Marker for Increasing the Implantation Success in the Infertile Women.
作者信息
Tajeddin Nahid, Ahadi Ali Mohammad, Javadi Gholamreza, Ayat Hoda
机构信息
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Genetics, Faculty of Science, University of Shahrekord, Shahrekord, Chaharmahal and Bakhtiari Province, Iran.
出版信息
Int J Prev Med. 2020 Feb 17;11:18. doi: 10.4103/ijpvm.IJPVM_398_18. eCollection 2020.
BACKGROUND
There are numerous couples worldwide currently suffering from infertility. Several factors, including genetic abnormalities are involved in infertility. In this study, we investigated the expression of myc gene in uterine tissue of infertile women. The protein encoded by this gene is one of the important transcription factors involved in the expression of many genes in the embryonic growth, and development pathways.
METHODS
There are about 45 samples of uterine tissue from women with primary and secondary infertility were involved in this study. After extracting RNA and synthesizing cDNA, using specific primers for the myc gene and the beta-actin gene (as an internal control), gene expression was evaluated by Real-time RT-PCR method.
RESULTS
The results of myc gene expression analysis showed no significant pattern between the affected and healthy women, however decreasing of its expression should not be rejected.
CONCLUSIONS
This study is the first report about myc gene expression and its relation with the primary and secondary infertility. Myc gene expression study at different times of sexual period of infertile woman is suggested. Also, we proposed here, as a preventive strategy, improvement of the expression level of myc gene by some methods, such as hormone therapy, can increase the implantation success in the infertile women.
背景
目前全球有众多夫妇遭受不孕不育问题。包括基因异常在内的多种因素与不孕不育有关。在本研究中，我们调查了不孕女性子宫组织中myc基因的表达。该基因编码的蛋白质是参与胚胎生长和发育途径中许多基因表达的重要转录因子之一。
方法
本研究纳入了约45例原发性和继发性不孕女性的子宫组织样本。提取RNA并合成cDNA后，使用针对myc基因和β-肌动蛋白基因（作为内对照）的特异性引物，通过实时逆转录聚合酶链反应方法评估基因表达。
结果
myc基因表达分析结果显示，患病女性和健康女性之间没有显著模式，但其表达降低也不能被排除。
结论
本研究是关于myc基因表达及其与原发性和继发性不孕不育关系的首次报道。建议在不孕女性性周期的不同时间进行myc基因表达研究。此外，我们在此提出，作为一种预防策略，通过激素治疗等一些方法提高myc基因的表达水平，可以提高不孕女性的着床成功率。
Zotero 插件