Karakişi Sedat Ozan, Hemşinli Doğuş, Ergene Şaban, Mercantepe Tolga, Tümkaya Levent, Yılmaz Adnan
Department of Cardiovascular Surgery, Recep Tayyip Erdoğan University Medical Faculty, Rize, Turkey.
Department of Histology and Embryology, Recep Tayyip Erdoğan University Medical Faculty, Rize, Turkey.
Turk Gogus Kalp Damar Cerrahisi Derg. 2020 Jan 23;28(1):63-69. doi: 10.5606/tgkdc.dergisi.2020.18389. eCollection 2020 Jan.
The aim of this study was to investigate the potential protective effect of whortleberry by examining the effects on heart tissue at the molecular level of ischemia-reperfusion injury caused by surgical repair of a ruptured abdominal aortic aneurysm.
Between May 2018 and February 2019, a total of 32 male Sprague-Dawley rats were randomly assigned into control, sham (ischemia-reperfusion+glycerol), ischemia-reperfusion, and ischemia-reperfusion+whortleberry groups. Hypovolemic shock was applied to the rats in the ischemia-reperfusion groups for one hour. The abdominal aorta was explored following midline laparotomy and atraumatic microvascular clamps were applied from the infrarenal level. Following one-hour ischemia, the clamps were removed, and reperfusion was established for two hours. In the sham group, intraperitoneal glycerol once daily was applied five days before surgery. In the whortleberry group, whortleberry treatment was administered via the intraperitoneal route five days before ischemia-reperfusion.
The ischemia-reperfusion group exhibited a decrease in the glutathione levels and an increase in the malondialdehyde levels (p<0.01 and p<0.01, respectively). We also observed an increase in the caspase-3 positivity in cardiac myofibrils (p<0.01). Whortleberry administration lowered both malondialdehyde levels and numerical density of caspase-3 positive cardiac myofibrils, while increasing the heart tissue glutathione levels, compared to the ischemia-reperfusion alone group (p<0.01, p=0.011, and p=0.011, respectively).
Whortleberry may be beneficial in preventing cardiac tissue damage caused by ischemia-reperfusion in the surgical repair of ruptured abdominal aortic aneurysms.
本研究的目的是通过研究越橘对腹主动脉瘤破裂手术修复所致缺血再灌注损伤心脏组织分子水平的影响，来探究其潜在的保护作用。
在2018年5月至2019年2月期间，总共32只雄性Sprague-Dawley大鼠被随机分为对照组、假手术组（缺血再灌注+甘油）、缺血再灌注组和缺血再灌注+越橘组。对缺血再灌注组的大鼠施加一小时的低血容量性休克。经中线剖腹术后探查腹主动脉，并从肾下水平应用无创伤微血管夹。缺血一小时后，移除夹子并建立两小时的再灌注。在假手术组中，术前五天每天腹腔注射一次甘油。在越橘组中，在缺血再灌注前五天通过腹腔途径给予越橘治疗。
缺血再灌注组的谷胱甘肽水平降低，丙二醛水平升高（分别为p<0.01和p<0.01）。我们还观察到心肌原纤维中caspase-3阳性率增加（p<0.01）。与单纯缺血再灌注组相比，给予越橘可降低丙二醛水平和caspase-3阳性心肌原纤维的数值密度，同时增加心脏组织谷胱甘肽水平（分别为p<0.01、p=0.011和p=0.011）。
在腹主动脉瘤破裂手术修复中，越橘可能有助于预防缺血再灌注引起的心脏组织损伤。
