Key Laboratory of New Materials and Facilities for Rural Renewable Energy of China's Ministry of Agriculture and Rural Affairs, College of Mechanical & Electrical Engineering, Henan Agricultural University, Zhengzhou, China.
Henan International Joint Laboratory of Biomass Energy and Nanomaterials, Henan Agricultural University, Zhengzhou, China.
Bioengineered. 2020 Dec;11(1):397-407. doi: 10.1080/21655979.2020.1736736.
The fur is hard to decompose during the fermentation process of diseased swine carcasses. In order to enhance the enzymolysis of pigskin, the ultrasonic was proposed to use during the process of the enzymatic hydrolysis. The response surface optimization experiments were carried out with the DH (degree of hydrolysis) as the response value and the optimum conditions for enzymatic hydrolysis were determined. Based the optimum conditions, orthogonal experiments were carried out with ultrasonic frequency, power and time as variables, and optimal ultrasonic parameters were obtained. Without the assistance of ultrasonic, the descending order of influence factors on DH was, temperature＞SC(Substrate concentration)＞RES(The ratio of enzyme to substrate)＞pH. Moreover, the DH value is of 10.42% under the following optimal conditions: RES is of 16,006 U/g, the temperature is of 48.92°C, the SC is of 59.76 g/L and pH is of 10.43. Frequency has the greatest effect on DH, followed by power, and finally time. The optimum hydrolysis time is of 5 h, and the DH is of 22.94% were obtained under the following optimum ultrasonic pretreatment conditions: frequency combination is of (20,40,40), power is of 600 W and time is of 25 min. Comparing with the group without ultrasonic pretreatment, the DH for the ultrasonic assistance increased by 4%, the hydrolysis time was shorten by 3 h, and the total amino acids increased by 15.98%.
病猪尸体在发酵过程中，其皮毛很难分解。为了提高猪皮的酶解效率，提出了在酶解过程中使用超声波。以水解度（DH）为响应值，进行响应面优化实验，确定酶解的最佳条件。在最佳条件下，以超声波频率、功率和时间为变量进行正交实验，得出最佳的超声波参数。在没有超声波辅助的情况下，影响 DH 的因素按降序排列为：温度＞底物浓度（SC）＞酶与底物的比例（RES）＞pH。此外，在以下最佳条件下，DH 值为 10.42%：RES 为 16,006 U/g，温度为 48.92°C，SC 为 59.76 g/L，pH 为 10.43。频率对 DH 的影响最大，其次是功率，最后是时间。最佳水解时间为 5 h，在以下最佳超声波预处理条件下，DH 值为 22.94%：频率组合为（20,40,40），功率为 600 W，时间为 25 min。与未经超声波预处理的组相比，超声波辅助处理的 DH 增加了 4%，水解时间缩短了 3 h，总氨基酸增加了 15.98%。
