Department of Gastrointestinal Surgery, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Science, Jinan, China.
Internal Medicine-Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China.
Curr Pharm Des. 2020;26(22):2657-2660. doi: 10.2174/1381612826666200316155252.
In the era of precision medicine, establishing a patient-derived cell model is crucial, whether in vitro or in vivo. Compared to the traditional cell lines, patient-derived primary cells represent precise genetic features from specific patients, but poor proliferative activity of human primary cells restricts their popular application. Conditional reprogramming (CR) is a new cell culture technique to achieve rapid growth of patient-derived cells in vitro, making it possible to identify the individual difference and screen drugs sensitivity. In this review, we will summarize the application and limitation of CR in tumor and inflammatory-related diseases, indicating the prospect of this technique for preclinical research.
在精准医学时代，建立患者来源的细胞模型至关重要，无论是体外还是体内。与传统的细胞系相比，患者来源的原代细胞代表了特定患者的精确遗传特征，但人类原代细胞增殖活性差限制了其广泛应用。条件重编程（CR）是一种新的细胞培养技术，可实现患者来源细胞的体外快速生长，从而有可能识别个体差异和筛选药物敏感性。在这篇综述中，我们将总结 CR 在肿瘤和炎症相关疾病中的应用和局限性，表明该技术在临床前研究中的前景。
