el-Gamal B A, Perry L A, Landon J
Department of Chemical Pathology, St Bartholomew's Hospital, London, England, UK.
Scand J Clin Lab Invest. 1988 Oct;48(6):513-8. doi: 10.3109/00365518809085766.
We describe a direct, rapid, sensitive and highly specific radioimmunoassay for determining serum levels of 17-hydroxyprogesterone. It is based on the use of highly specific sheep antiserum, 125I-labelled tracer, dextran-coated charcoal to separate the antibody bound and free fractions, and sodium salicylate to eliminate interference from endogenous binding proteins in serum. Intra- and interassay coefficients of variation are less than 8% and recovery is satisfactory. Sensitivity is 3.5 fmol per assay tube (0.14 nmol/l). Results correlate closely with those of an established technique using 3H-labelled steroid after initial solvent extraction and column chromatography of samples (y = 1.02x - 0.16; r = 0.998). The values found for serum from normal adult subjects ranged from 1.0 to 12.1 nmol/l while those from treated and untreated patients with congenital adrenal hyperplasia due to 21-hydroxylase deficiency were 1.4-18.4 and 27.3-650 nmol/l, respectively.
我们描述了一种用于测定血清17-羟孕酮水平的直接、快速、灵敏且高度特异的放射免疫分析方法。该方法基于使用高度特异的绵羊抗血清、125I标记的示踪剂、葡聚糖包被活性炭来分离抗体结合部分和游离部分，以及水杨酸钠来消除血清中内源性结合蛋白的干扰。批内和批间变异系数均小于8%，回收率良好。灵敏度为每个测定管3.5 fmol（0.14 nmol/L）。在对样品进行初始溶剂萃取和柱色谱分析后，结果与使用3H标记类固醇的既定技术的结果密切相关（y = 1.02x - 0.16；r = 0.998）。正常成年受试者血清的值范围为1.0至12.1 nmol/L，而因21-羟化酶缺乏导致先天性肾上腺皮质增生的经治疗和未经治疗患者的血清值分别为1.4 - 18.4 nmol/L和27.3 - 650 nmol/L。
