el-Gamal B A, Eremin S A, Smith D S, Landon J
Department of Chemical Pathology, St Bartholomew's Hospital, London, UK.
Ann Clin Biochem. 1988 Jan;25 ( Pt 1):35-41. doi: 10.1177/000456328802500104.
A direct, rapid and highly specific fluoroimmunoassay for determining serum levels of 17-hydroxyprogesterone has been developed. It is based on the use of a sheep antiserum covalently coupled to magnetisable particles and fluorescein-labelled steroid. Sodium salicylate is employed to eliminate interference from endogenous binding proteins in serum. The sensitivity of 0.5 nmol/L is adequate for clinical purposes. Analytical recovery, linearity and precision are satisfactory and the results obtained correlate closely with those of an established radioimmuno-assay using 3H-labelled steroid and the same antiserum after initial sample extraction and chromatography. The values found for serum from normal adult subjects ranged from 1.0 to 12.6 nmol/L while those from treated and untreated patients with congenital adrenal hyperplasia due to 21-hydroxylase deficiency were 1.5 to 19.0 and 28.0 to 655 nmol/L, respectively.
已开发出一种用于测定血清17 - 羟孕酮水平的直接、快速且高度特异的荧光免疫测定法。它基于使用与可磁化颗粒共价偶联的绵羊抗血清和荧光素标记的类固醇。采用水杨酸钠消除血清中内源性结合蛋白的干扰。0.5 nmol/L的灵敏度足以满足临床目的。分析回收率、线性和精密度令人满意，并且在对初始样品进行提取和色谱分离后，所获得的结果与使用3H标记类固醇和相同抗血清的既定放射免疫测定法的结果密切相关。正常成年受试者血清的值范围为1.0至12.6 nmol/L，而因21 - 羟化酶缺乏导致先天性肾上腺皮质增生的治疗和未治疗患者的血清值分别为1.5至19.0 nmol/L和28.0至655 nmol/L。
