Prado Marcelle SanJuan Ganem, de Jesus Mirthz Lemos, de Goes Thaline Cunha, Mendonça Lucilla Silva Oliveira, Kaneto Carla Martins
Department of Health Science, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil.
Department of Biological Science, Universidade Estadual de Santa Cruz, Rodovia Jorge Amado, Km16, Ilhéus, BA, 45662-900, Brazil.
BMC Res Notes. 2020 Mar 16;13(1):155. doi: 10.1186/s13104-020-05001-9.
To evaluate the expression of a set of miRNAs to identify differentially expressed miRNAs that might be considered reliable biomarkers on Diabetic Retinopathy (DR) blood samples.
Expression levels of MiR-320a, MiR-342-3p, MiR-155, MiR-99a, MiR-29a and MiR-27b were analyzed in 60 healthy controls, 48 Diabetes Melitus (DM) without DR patients and 62 DR patients by qRT-PCR. MiR-320a was shown to be downregulated in the plasma of DR patients compared with DM patients without DR and healthy subjects. Target genes were predicted using miRWalk3.0, miR targeting data and target gene interaction data were imported to Cytoscape to visualize and merge networks and top ranked predicted genes were run through Ontology Genes to perform enrichment analysis on gene sets and classification system to identify biological processes and reactome pathways associated with DR. Highly scored target genes of miR-320a were categorized for various biological processes, including negative regulation of cell aging, negative regulation of cellular protein metabolic process and regulation of cellular response to stress that are critical to the development of DR. Our findings suggest that MiR-320a may have a role in the pathogenesis of DR and may represent novel biomarkers for this disease.
评估一组微小RNA（miRNA）的表达，以鉴定在糖尿病视网膜病变（DR）血样中可能被视为可靠生物标志物的差异表达miRNA。
通过qRT-PCR分析了60名健康对照者、48名无DR的糖尿病（DM）患者和62名DR患者中MiR-320a、MiR-342-3p、MiR-155、MiR-99a、MiR-29a和MiR-27b的表达水平。与无DR的DM患者和健康受试者相比，DR患者血浆中MiR-320a表达下调。使用miRWalk3.0预测靶基因，将miR靶向数据和靶基因相互作用数据导入Cytoscape以可视化和合并网络，并对排名靠前的预测基因通过本体基因进行基因集富集分析和分类系统分析，以识别与DR相关的生物学过程和反应组途径。miR-320a的高分靶基因被归类为各种生物学过程类别，包括对细胞衰老的负调控、细胞蛋白质代谢过程的负调控以及对细胞应激反应的调控，这些对DR的发展至关重要。我们的研究结果表明，MiR-320a可能在DR的发病机制中起作用，并且可能代表该疾病的新型生物标志物。
