AgroParisTech, Paris, France.
Department of Animal Breeding and Genetics, Swedish University of Agriculture Science, Uppsala, Sweden.
Vet Res. 2020 Mar 16;51(1):44. doi: 10.1186/s13567-020-00768-y.
Gastrointestinal nematode (GIN) infections are one of the major constraints for grazing sheep and goat production worldwide. Genetic selection for resistant animals is a promising control strategy. Whole-transcriptome analysis via RNA-sequencing (RNA-seq) provides knowledge of the mechanisms responsible for complex traits such as resistance to GIN infections. In this study, we used RNA-seq to monitor the dynamics of the response of the abomasal mucosa of Creole goat kids infected with Haemonchus contortus by comparing resistant and susceptible genotypes. A total of 8 cannulated kids, 4 susceptible and 4 resistant to GIN, were infected twice with 10 000 L3 H. contortus. During the second infection, abomasal mucosal biopsies were collected at 0, 8, 15 and 35 days post-infection (dpi) from all kids for RNA-seq analysis. The resistant animals showed early activation of biological processes related to the immune response. The top 20 canonical pathways of differentially expressed genes for different comparison showed activation of the immune response through many relevant pathways including the Th1 response. Interestingly, our results showed a simultaneous time series activation of Th2 related genes in resistant compared to susceptible kids.
胃肠道线虫（GIN）感染是全球放牧绵羊和山羊生产的主要限制因素之一。对抗性动物进行遗传选择是一种有前途的控制策略。通过 RNA 测序（RNA-seq）进行的全转录组分析为研究抵抗 GIN 感染等复杂性状的机制提供了知识。在这项研究中，我们使用 RNA-seq 来监测感染捻转血矛线虫的克里奥尔山羊幼崽胃黏膜反应的动态，通过比较抗性和易感基因型。共有 8 只插管的幼崽，4 只为 GIN 敏感，4 只为 GIN 抗性，被两次感染了 10,000 个捻转血矛线虫 L3。在第二次感染期间，从所有幼崽中在感染后 0、8、15 和 35 天收集胃黏膜活检进行 RNA-seq 分析。抗性动物表现出与免疫反应相关的生物学过程的早期激活。不同比较的差异表达基因的前 20 个经典途径显示通过许多相关途径激活免疫反应，包括 Th1 反应。有趣的是，我们的结果显示，与易感幼崽相比，抗性幼崽同时呈现 Th2 相关基因的时间序列激活。
