Afanas'ev V N, Korol' B A, Matylevich N P, Pechatnikov V A, Umanskiĭ S R
Tsitologiia. 1988 Sep;30(9):1108-16.
It is shown that colchicine injection at doses higher than 1 mg/kg of animal weight induces cell death in thymus, spleen, bone marrow and intestine mucosa. The cell death is accompanied by a regular internucleosomal cleavage of nuclear DNA and by the elimination of the formed fragments from cells. Both the processes begin after a 1.5 hour lag-period and proceed before the outer membrane permeability for supravital dyes increases. DNA degradation is prevented by the inhibitor of protein synthesis cycloheximide. Cytochalasin B does not induce chromatin degradation or cell death and has no effect on radiation death of lymphocytes. A possible role of microtubule destruction as a switch-on mechanism of DNA degradation and cell death is discussed.
结果表明，以高于每千克动物体重1毫克的剂量注射秋水仙碱会诱导胸腺、脾脏、骨髓和肠黏膜中的细胞死亡。细胞死亡伴随着核DNA规则的核小体间切割以及细胞内形成片段的清除。这两个过程在1.5小时的延迟期后开始，并在超活染料的外膜通透性增加之前进行。蛋白质合成抑制剂环己酰亚胺可阻止DNA降解。细胞松弛素B不会诱导染色质降解或细胞死亡，对淋巴细胞的辐射死亡也没有影响。文中讨论了微管破坏作为DNA降解和细胞死亡启动机制的可能作用。
