Department of Diabetes, School of Life Course Sciences, Faculty of Life Science and Medicine, King's College London, London, UK.
Methods Mol Biol. 2020;2128:207-216. doi: 10.1007/978-1-0716-0385-7_14.
Type 2 diabetes is characterized by glucose intolerance, caused by insulin resistance in peripheral metabolic tissues and by impaired glucose-stimulated insulin secretion, the hallmark of beta-cell dysfunction. The glucose tolerance test is used in clinic and research to identify individuals with impaired glucose tolerance and overt type 2 diabetes. It is the most routinely used physiological test for first pass assessment of glucose homeostasis in rodents because of its simplicity. The GTT measures changes in blood glucose concentration over a 2-h period following the administration of a bolus of glucose. However, this simplicity belies several important considerations which need to be addressed, to aid reproducibility and produce interpretable data. Here, we describe in detail how to perform a GTT using four different routes of glucose administration: intraperitoneal, oral, voluntary oral, and intravenous.
2 型糖尿病的特征是葡萄糖耐量受损，这是由外周代谢组织的胰岛素抵抗和葡萄糖刺激的胰岛素分泌受损引起的，这是β细胞功能障碍的标志。葡萄糖耐量试验用于临床和研究中，以确定葡萄糖耐量受损和明显 2 型糖尿病患者。由于其简单性，它是啮齿动物中葡萄糖稳态首次评估最常用的生理测试。GTT 测量在给予葡萄糖冲击后 2 小时内血糖浓度的变化。然而，这种简单性掩盖了一些需要解决的重要问题，以提高可重复性并产生可解释的数据。在这里，我们详细描述了如何使用四种不同的葡萄糖给药途径：腹腔内、口服、自愿口服和静脉内来进行 GTT。
