Ann Ital Chir. 2020;91:131-136.
We aimed to show whether ischemia reperfusion (I/R) injury causes damage on brain or not, and whether thymoquinone and silymarin, as antioxidant and anti-inflammatory herbs, have beneficial effects on this damage or not.
Forty Wistar albino rats were carried out and were randomized to 4 groups with equal numbers (n=10): sham group, implemented of only anesthesia; control group, implemented of anesthesia and I/R injury; silymarin group, implemented of anesthesia and I/R injury and treated with a dose of 200 milligram/kg silymarin ip and thymoquinone group, implemented of anesthesia and I/R injury and treated with a dose of 20 mg/kg thymoquinone. Serum lipid hydroperoxide (LOOH) and total free sulfhydryl (Sh) levels were determined. Light microscopy was used to evaluate histological changes in brain tissue.
Serum LOOH levels (0.21 ± 0.04 for control group, 0.29 ± 0.01 for sham group, 0.23 ± 0.09 for silymarin group, 0.29 ± 0.09 for thymoquinone group) were significantly higher and Sh levels (10.74 ± 1.71 for control group, 6.82 ± 0.24 for sham group, 9.12 ± 1.04 for silymarin group, 8.41 ± 1.12 for thymoquinone group) were significantly lower in control, silymarin and thymoquinone groups compared to control group (p<0.05 for all). According to the histopathologic damage score assessment, it was seen that the damage decreased significantly in the silymarin and the thymoquinone groups.
We showed that tissue damage also occurs in brain following the ischemia reperfusion. It was shown that thymoquinone and silymarin is quite effective in preventing this damage.
Brain, Hydroperoxide levels, Ischemia reperfusion injury, Sulfhydryl levels, Silymarin, Thymoquinone.
本研究旨在探讨缺血再灌注（I/R）损伤是否会对大脑造成损伤，以及抗氧化和抗炎草药如胸腺醌和水飞蓟素是否对此类损伤具有有益作用。
将 40 只 Wistar 白化大鼠进行分组，每组 10 只：假手术组，仅进行麻醉；对照组，进行麻醉和 I/R 损伤；水飞蓟素组，进行麻醉和 I/R 损伤，并给予 200 毫克/千克水飞蓟素腹腔注射；胸腺醌组，进行麻醉和 I/R 损伤，并给予 20 毫克/千克胸腺醌。测定血清脂质过氧化物（LOOH）和总游离巯基（Sh）水平。采用光镜观察脑组织的组织学变化。
与对照组（0.21±0.04）相比，血清 LOOH 水平（0.29±0.01，假手术组；0.23±0.09，水飞蓟素组；0.29±0.09，胸腺醌组）显著升高，Sh 水平（10.74±1.71，对照组；6.82±0.24，假手术组；9.12±1.04，水飞蓟素组；8.41±1.12，胸腺醌组）显著降低（p<0.05）。根据组织病理学损伤评分评估，发现水飞蓟素组和胸腺醌组的损伤明显减轻。
我们发现，缺血再灌注后大脑也会发生组织损伤。胸腺醌和水飞蓟素在预防这种损伤方面非常有效。
脑、过氧化物水平、缺血再灌注损伤、巯基水平、水飞蓟素、胸腺醌。
