Department of Cardiovascular Surgery, Medical Faculty, Harran University, Şanliurfa, Turkey.
Department of Biochemistry, Medical Promotion and Marketing Program, Health Services Vocational School, Harran University, Şanliurfa, Turkey.
Braz J Cardiovasc Surg. 2022 Dec 1;37(6):807-813. doi: 10.21470/1678-9741-2021-0462.
Medical improvements are needed to prevent ischemia-reperfusion injury in thoracoabdominal aortic surgery. The aim of this study was to determine the antioxidant effects of thymoquinone, silymarin, and curcumin against ischemia-reperfusion injury associated with abdominal aorta.
Twenty-five Wistar albino rats were included in the study. Sham, control, and treatment (thymoquinone, silymarin, and curcumin) groups were set in equal numbers. Ischemia-reperfusion was applied by clamping (120 minutes) and de-clamping (60 minutes) the infrarenal aorta of all groups, except the sham group. Before reperfusion, thymoquinone, silymarin, and curcumin were given intraperitoneally to the treatment groups. After reperfusion, blood samples were taken from the right ventricle. Total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were studied in serum samples and histopathological examination was performed on the gastrocnemius muscle.
There was a significant difference in TOS and OSI values between the control and sham groups. Both values were found higher in the control group than in the sham group (P<0.05). OSI values were found to be lower in the thymoquinone group compared to the control group (P<0.05). All three parameters were found to be lower in the silymarin group than in the control group (P<0.05). TAS and TOS levels were found to be higher in the curcumin group than in the control group (P<0.05). There was no histopathological difference between the groups.
Silymarin and thymoquinone administration decreases oxidative stress in experimental aortic ischemia-reperfusion injury. Antioxidant effect of curcumin was lower than silymarin and thymoquinone.
胸主动脉腹主动脉手术中需要进行医学改进以防止缺血再灌注损伤。本研究的目的是确定百里醌、水飞蓟素和姜黄素对腹部主动脉缺血再灌注损伤的抗氧化作用。
将 25 只 Wistar 白化大鼠纳入研究。设置相同数量的假手术、对照组和治疗组（百里醌、水飞蓟素和姜黄素）。除假手术组外，所有组的腹主动脉均进行夹闭（120 分钟）和松解（60 分钟）的缺血再灌注。再灌注前，将百里醌、水飞蓟素和姜黄素腹腔内给予治疗组。再灌注后，从右心室取血样。研究血清样本中的总抗氧化状态（TAS）、总氧化状态（TOS）和氧化应激指数（OSI），并对腓肠肌进行组织病理学检查。
对照组和假手术组之间 TOS 和 OSI 值存在显著差异。对照组的值均高于假手术组（P<0.05）。与对照组相比，百里醌组的 OSI 值较低（P<0.05）。与对照组相比，水飞蓟素组的所有三个参数均较低（P<0.05）。与对照组相比，姜黄素组的 TAS 和 TOS 水平较高（P<0.05）。各组之间的组织病理学无差异。
水飞蓟素和百里醌给药可降低实验性主动脉缺血再灌注损伤中的氧化应激。姜黄素的抗氧化作用低于水飞蓟素和百里醌。
