Li Man, Wang Fang, Du Juan, Wang Lijuan, Zhang Jianzhong, Ding Xiaolan
Department of Dermatology, Peking University People's Hospital, Beijing, China.
Indian J Dermatol. 2020 Mar-Apr;65(2):85-91. doi: 10.4103/ijd.IJD_593_18.
Oxidative stress is implicated in the pathogenesis of vitiligo. The function of DJ-1 in oxidative damage of melanocytes is still elusive.
The aim of this study was to investigate the role of DJ-1 in oxidative damage of melanocytes.
The expression of DJ-1 in melanocytes was studied by reverse transcription-quantitative polymerase chain reaction and Western blot. Short-interfering RNAs (siRNA) were employed to downregulate DJ-1. The cells were pooled into three groups: mock group (cells with transfection reagent), negative control (NC) group (negative siRNA control), and siRNA group. After HO treatment for 24 h, the morphological changes, cell viability, apoptosis, intracellular reactive oxygen species (ROS) levels, mitochondrial membrane potential (MMP), and mitochondrial respiration were measured in different groups.
DJ-1 was highly expressed in PIG1 melanocytes. DJ-1 knockdown rendered PIG1 melanocytes more susceptible to oxidative stress. Loss of DJ-1 led to apoptosis of PIG1 cells by impairing the function of mitochondria, including morphological abnormalities, ROS accumulation, depolarization of MMP, less adenosine-triphosphate (ATP) production, and less proton leak.
DJ-1 plays a role in maintaining the antioxidative capacity in epidermal melanocytes.
氧化应激与白癜风的发病机制有关。DJ-1在黑素细胞氧化损伤中的作用仍不清楚。
本研究旨在探讨DJ-1在黑素细胞氧化损伤中的作用。
采用逆转录-定量聚合酶链反应和蛋白质免疫印迹法研究DJ-1在黑素细胞中的表达。使用小干扰RNA（siRNA）下调DJ-1。将细胞分为三组：模拟组（转染试剂处理的细胞）、阴性对照组（阴性siRNA对照）和siRNA组。在过氧化氢（HO）处理24小时后，检测不同组细胞的形态变化、细胞活力、凋亡、细胞内活性氧（ROS）水平、线粒体膜电位（MMP）和线粒体呼吸。
DJ-1在PIG1黑素细胞中高表达。DJ-1基因敲低使PIG1黑素细胞对氧化应激更敏感。DJ-1缺失通过损害线粒体功能导致PIG1细胞凋亡，包括形态异常、ROS积累、MMP去极化、三磷酸腺苷（ATP）生成减少和质子泄漏减少。
DJ-1在维持表皮黑素细胞的抗氧化能力中起作用。
