Kotlovskiĭ Iu V, Grishanova A Iu, Mishin V M, Bachmanova G I
Vopr Med Khim. 1988 Sep-Oct;34(5):14-7.
Rat liver microsomal carboxylesterase catalyzed hydrolysis of butyl methacrylate (BMA), butyl acrylate (BA), methyl acrylate (MA) and methyl methacrylate (MMA). The rate of butanol formation from BA and BMA was 4-fold higher as compared with methanol production from MA and MMA. Methanol was oxidized to formaldehyde at the next step of microsomal metabolism. The rate of methanol oxidation was increased 2.2-fold after addition of the NADPH-generating system into the incubation medium. In the mixture containing MMA, NADPH-generating system and liver microsomes, preinduced with phenobarbital or methyl cholanthrene, the rate of formaldehyde generation was increased 7- and 2-fold, respectively, as compared with the analogous experiments involving liver microsomes of control rats. Production of methanol and generation of formaldehyde were not detected after incubation of MMA with inactivated microsomes. The microsomal MMA-methanol oxidizing system was inhibited by CO or after addition of microsomes from rats pretreated with CoCL2. Sodium azide (0.05 mM) decreased 8-fold the rate of formaldehyde generation.
大鼠肝脏微粒体羧酸酯酶催化甲基丙烯酸丁酯（BMA）、丙烯酸丁酯（BA）、丙烯酸甲酯（MA）和甲基丙烯酸甲酯（MMA）的水解。与由MA和MMA生成甲醇相比，由BA和BMA生成丁醇的速率高4倍。在微粒体代谢的下一步，甲醇被氧化为甲醛。向孵育介质中添加NADPH生成系统后，甲醇氧化速率提高了2.2倍。在含有MMA、NADPH生成系统和经苯巴比妥或甲基胆蒽预诱导的肝脏微粒体的混合物中，与涉及对照大鼠肝脏微粒体的类似实验相比，甲醛生成速率分别提高了7倍和2倍。用灭活的微粒体孵育MMA后，未检测到甲醇的生成和甲醛的产生。微粒体MMA - 甲醇氧化系统可被CO抑制，或在添加经CoCl2预处理的大鼠的微粒体后被抑制。叠氮化钠（0.05 mM）使甲醛生成速率降低了8倍。
