[Effect of acrylates on the level and isozyme composition of cytochrome P-450 in rat liver microsomes].

Content of cytochrome P-450 was decreased in rat liver microsomes, pretreated with phenobarbital, after incubation with acrylamide, methyl methacrylate and butyl methacrylate in presence of NADPH in vitro. The phenomenon was not observed in the analogous experiments but carried out without NADPH, thus suggesting the microsomal metabolism of the acrylates. At the same time, after intraperitoneal administration of acrylamide 0.4 mmole/kg, methyl methacrylate 6.0 mmole/kg and butyl methacrylate 9.0 mmole/kg within 3 days into intact and protected with phenobarbital rats alterations in total content of cytochrome P-450 were not observed. All the acrylates studied exhibited the slight inducing effect on cytochrome P-450b but did not affect the cytochrome P-450c. Methyl- and butyl methacrylates decreased distinctly the content of cytochrome P-450 isoenzyme with molecular mass of 48,000 daltons.