Earth and Life Institute, Université catholique de Louvain (UCLouvain), Louvain-la-Neuve, Belgium.
Centre Indépendant de Promotion Fourragère (CIPF), Louvain-la-Neuve, Belgium.
J Appl Microbiol. 2020 Sep;129(3):637-651. doi: 10.1111/jam.14641. Epub 2020 Apr 8.
The objective of this work was to identify a fungal strain showing potential biocontrol abilities against two Fusarium damping-off agents and to test it as a Biological Control Agent (BCA) in maize seed coating under field conditions.
A collection of native fungal strains associated with maize in Belgium was screened for antagonistic potential against Fusarium avenaceum and Fusarium culmorum. The strain with highest biocontrol potential was identified as an endophytic Trichoderma atroviride BC0584. In greenhouse, it significantly improves the emergence of seedlings infected by F. avenaceum or F. culmorum pathogens. In most field trials carried out during the season 2017, it significantly increased the emergence rate of infected seedlings compared to untreated seeds. One slurriable powder formulation allows BCA conidia to survive over a 6-month storage period at 4°C.
The fungal BC0584 strain is a promising BCA that could be an alternative to synthetic fungicides. It is adapted to local environmental conditions, is easily and cheaply produced and can be stored in a low-cost formulation.
In Belgium, this is the first study to use a T. atroviride native strain against Fusarium damping-off on maize crop. Modes of action and required conditions for ensuring high biocontrol activity in the field have still to be investigated.
本研究的目的是鉴定一种具有潜在生防能力的真菌菌株，以防治两种镰刀菌猝倒病病原菌，并在田间条件下作为生物防治剂（BCA）对玉米种子进行包衣处理。
本研究对与比利时玉米相关的天然真菌菌株进行了筛选，以评估其对尖孢镰刀菌和禾谷镰刀菌的拮抗潜力。具有最高生防潜力的菌株被鉴定为内生拟青霉 BC0584。温室条件下，它可显著提高受尖孢镰刀菌或禾谷镰刀菌病原菌感染的幼苗的出苗率。在 2017 年进行的大多数田间试验中，与未处理种子相比，它显著提高了受感染幼苗的出苗率。一种可湿性粉剂配方可使 BCA 分生孢子在 4°C 下储存 6 个月而存活。
真菌 BC0584 菌株是一种很有前途的生物防治剂，可替代合成杀菌剂。它适应于当地的环境条件，易于生产且成本低廉，可在低成本配方中储存。
在比利时，这是首次使用本土拟青霉菌株防治玉米上的镰刀菌猝倒病。生防活性在田间的作用方式和所需条件仍有待研究。
