Department of Plant Pathology, North Dakota State University, Fargo, ND 58108-6050, U.S.A.
School of Life and Medical Sciences, University of Hertfordshire, Hatfield, AL10 9AB, U.K.
Plant Dis. 2020 May;104(5):1312-1317. doi: 10.1094/PDIS-09-19-1895-RE. Epub 2020 Mar 16.
Minnesota and North Dakota combined contain 55% of the sugar beet production area in the United States, contributing to 49% of the nation's sugar beet production in 2018. Fusarium diseases caused by f. and on sugar beet can cause significant reduction in both root yield and sucrose concentration and purity. The objective of this research was to identify an alternative artificial inoculation method to induce Fusarium diseases on sugar beet leaves and roots caused by both spp. in greenhouse conditions to better aid in research efforts. We tested four inoculation methods, including barley to seed, barley to root, drenching, and cutting. and compared them with the conventional root-dipping inoculation method. The inoculation method of placing -colonized barley seed close to sugar beet seed (barley to seed) caused levels of symptom severities on both leaves and roots similar to the root-dipping method. Because the traditional root-dipping method involves a laborious transplant process, use of infected barley seed as inoculum may serve as an alternative method in the evaluation of host resistance and pathogen virulence among Fusarium diseases by spp. on sugar beet at the seed or seedling stage.
明尼苏达州和北达科他州的糖用甜菜种植区加起来占美国的 55%，为 2018 年美国 49%的糖用甜菜产量做出了贡献。镰刀菌属真菌引起的镰刀菌病害会导致甜菜根产量和蔗糖浓度及纯度显著降低。本研究的目的是在温室条件下找到一种替代的人工接种方法，以诱导由 spp. 引起的甜菜叶片和根部的镰刀菌病害，从而更好地辅助研究工作。我们测试了包括大麦种子接种、大麦根部接种、浸种和切段在内的 4 种接种方法，并将其与传统的根部浸蘸接种方法进行了比较。将 - 定殖的大麦种子靠近甜菜种子放置的接种方法（大麦种子接种）在叶片和根部引起的症状严重程度与根部浸蘸方法相似。由于传统的根部浸蘸方法涉及到繁琐的移栽过程，因此使用感染的大麦种子作为接种物可能是在种子或幼苗阶段评估 spp. 引起的甜菜镰刀菌病害的寄主抗性和病原菌毒力的替代方法。
