通过生物信息学分析研究 WNT 家族成员在脑胶质瘤中的表达谱和预后意义。
Expression profiles and prognostic significance of WNT family members in glioma via bioinformatic analysis.
机构信息
Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, P.R. China.
The First Clinical Medical Institute of Southern Medical University, Guangzhou 510515, P.R. China.
出版信息
Biosci Rep. 2020 Mar 27;40(3). doi: 10.1042/BSR20194255.
AIMS
The dysregulation and essential role of WNTs in glioma have been widely implicated. However, there is a paucity of literature on the expression status of all the 19 WNTs in glioma. Our study was aimed to evaluate the expression and prognostic values of the 19 WNTs in glioma.
METHODS
mRNA expression and clinical data were retrieved from the Cancer Genome Atlas (TCGA) database, Chinese Glioma Genome Atlas (CGGA), GTEx and ONCOMINE databases. The 50 frequent neighbor genes of WNT5A and WNT10B were shown with PPI network, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses.
RESULTS
We found that the mRNA expression of WNT5A was significantly higher in glioma; however, the WNT10B expression was significantly lower in glioma. Furthermore, the expression of WNT5A and WNT10B was associated with the clinicopathology of glioma. The survival analysis revealed that the higher expressions of WNT5A and WNT16 were associated poor overall survival (OS) in patients with glioma. Conversely, overexpression of WNT3, WNT5B, and WNT10B was associated with better OS. Finally, Go and KEGG analysis revealed WNT5A was associated with multiple signal translations, and crucial oncogenes (EGFR and MDM2) and 2 important tumor suppressors (PTEN and IKN4a/ARF) were found closely correlated with WNT5A in glioma.
CONCLUSION
Among 19WNTs, WNT5A can serve as a candidate to diagnose and therapy glioma, while WNT10B might be valuable for anti-glioma research. The presumed direction was provided to explore the relation of WNTs signal and multiple pathways in glioma.
目的
WNT 信号在神经胶质瘤中的失调和重要作用已被广泛证实。然而，关于神经胶质瘤中所有 19 种 WNTs 的表达状态的文献却很少。本研究旨在评估 WNTs 在神经胶质瘤中的表达及其预后价值。
方法
从癌症基因组图谱（TCGA）数据库、中国神经胶质瘤基因组图谱（CGGA）、GTEx 和 ONCOMINE 数据库中检索 mRNA 表达和临床数据。WNT5A 和 WNT10B 的 50 个高频邻居基因通过 PPI 网络、基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析进行显示。
结果
我们发现 WNT5A 在神经胶质瘤中的 mRNA 表达明显升高，而 WNT10B 的表达则明显降低。此外，WNT5A 和 WNT10B 的表达与神经胶质瘤的临床病理特征有关。生存分析显示，WNT5A 和 WNT16 的高表达与神经胶质瘤患者的总生存期（OS）不良相关。相反，WNT3、WNT5B 和 WNT10B 的过表达与较好的 OS 相关。最后，GO 和 KEGG 分析显示 WNT5A 与多种信号转导有关，并且发现关键癌基因（EGFR 和 MDM2）和 2 个重要肿瘤抑制基因（PTEN 和 IKN4a/ARF）与神经胶质瘤中的 WNT5A 密切相关。
结论
在 19 种 WNTs 中，WNT5A 可作为诊断和治疗神经胶质瘤的候选物，而 WNT10B 可能对神经胶质瘤的研究有价值。为探讨 WNTs 信号与神经胶质瘤中多种途径的关系提供了初步方向。
Zotero 插件