School of Electrical Engineering, Purdue University, West Lafayette, IN, United States of America.
Birck Nanotechnology Center, Purdue University, West Lafayette, IN, United States of America.
PLoS One. 2020 Mar 17;15(3):e0229949. doi: 10.1371/journal.pone.0229949. eCollection 2020.
We present a two-tiered microchip system to capture and retrieve rare cells from blood samples with high purity. The first module of the system is a high throughput microfluidic interface that is used to immunomagnetically isolate targeted rare cells from whole blood, and discard > 99.999% of the unwanted leukocytes. The second module is a microwell array that furthers the purification by magnetically guiding each cell into a separate well concurrently, and allows individual retrieval of each cell. We demonstrate the design of the system as well as its characterization by experiments using model cell lines that represent circulating fetal trophoblasts. Our results show that single cells can be retrieved with efficiencies and purities as high as 100% within 145 mins.
我们提出了一种双层微芯片系统，用于从血液样本中以高纯度捕获和回收稀有细胞。该系统的第一个模块是高通量微流控界面，用于从全血中免疫磁分离靶向稀有细胞，并丢弃> 99.999％的不需要的白细胞。第二个模块是一个微孔阵列，通过同时将每个细胞引导到单独的孔中进行进一步的纯化，并允许单独回收每个细胞。我们通过使用代表循环胎儿滋养层的模型细胞系的实验来演示系统的设计及其特性。我们的结果表明，在 145 分钟内，单个细胞的回收率和纯度高达 100％。
