College of Geology and Environment, Xi'an University of Science and Technology, Xi'an 710054, China; Shaanxi Provincial Key Laboratory of Geological Support for Coal Green Exploitation, Xi'an 710054, China.
College of Geology and Environment, Xi'an University of Science and Technology, Xi'an 710054, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Jun 15;234:118233. doi: 10.1016/j.saa.2020.118233. Epub 2020 Mar 5.
Due to the own defects of natural enzymes, artificial simulated enzymes are always concerned. Here, the fabricated graphene oxide (GO)/AuNPs nanocomposite exhibits strong synergistic catalysis of peroxidase-mimicking enzymes in combination with the novel property of GO catalytic interface and AuNPs-mediated electron transfer. It can efficiently catalyze the oxidation of enzyme substrate TMB by hydrogen peroxide to form blue TMB oxide. Based on this, the rapid and highly sensitive colorimetric detection of hydrogen peroxide was achieved. Because of the wonderfully synergistic coupling catalysis from GO/AuNPs nanocomposites, the developed artificial enzyme has ultra-strong catalytic activity. For the detection of hydrogen peroxide, the detection limit of this colorimetric analysis is as low as 4.2 × 10 M, which is about 1-2 orders of magnitude lower than that of the assays using other single nanoparticles as nanozymes. And it shows high sensitivity. The catalytic oxidation of the prepared nanocomposites to TMB can be completed in minutes, and the response is extremely fast. Combined with the reaction of glucose and glucose oxidase, the colorimetric analysis also realizes the rapid and highly sensitive detection of glucose in human serum. The research results infer that the smart synergy is an effective way to improve the catalytic activity of mimic enzyme. Together with its simplicity in preparation, the GO/AuNPs nanocomposite has excellent development potential in biomedical detection and biosensor design.
由于天然酶自身的缺陷，人工模拟酶一直备受关注。在这里，所制备的氧化石墨烯（GO）/金纳米粒子（AuNPs）纳米复合材料结合了 GO 催化界面的新颖特性和 AuNPs 介导的电子转移，表现出了过氧化物酶模拟酶的强烈协同催化作用。它可以有效地催化过氧化氢酶底物 TMB 的氧化，形成蓝色 TMB 氧化物。基于此，可以实现对过氧化氢的快速高灵敏比色检测。由于 GO/AuNPs 纳米复合材料的奇妙协同偶联催化作用，所开发的人工酶具有超强的催化活性。对于过氧化氢的检测，该比色分析的检测限低至 4.2×10-7 M，比使用其他单纳米粒子作为纳米酶的测定方法低 1-2 个数量级。并且它具有高灵敏度。该纳米复合材料对 TMB 的催化氧化可以在几分钟内完成，响应非常迅速。结合葡萄糖和葡萄糖氧化酶的反应，比色分析还实现了对人血清中葡萄糖的快速高灵敏检测。研究结果推断，智能协同作用是提高模拟酶催化活性的有效途径。GO/AuNPs 纳米复合材料具有制备简单、灵敏度高的特点，在生物医学检测和生物传感器设计方面具有优异的发展潜力。
