Department of Chemistry, National Chung Hsing University, Taichung City 40227, Taiwan.
Department of Applied Chemistry, National Chiayi University, Chiayi City 60004, Taiwan.
Molecules. 2020 Mar 8;25(5):1217. doi: 10.3390/molecules25051217.
Adenosine -5'-triphosphate (ATP) plays a valuable role in metabolic activity to produce adequate energy in a biosystem. A high ATP/AMP ratio has a correlation with diabetes that induces suppression of AMP-activated protein kinase (AMPK). Matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry (MS) has outstanding potential in determining the ratio of several types of adenosine phosphates in a sample to rapidly understand the primary energy transfer in metabolism. Although MALDI is viewed as a soft ionization technique for MS analysis, excess photon energy might crack the phosphate bonds leading to misinterpretation of the ATP level. In this work, ionic liquid matrices (ILMs) were employed to reduce fragmentation and increase the detection efficiency during the MALDI process. This study demonstrated for the first time that 2,5-dihydroxybenzoic acid pyridine (DHBP) is one of the most effective matrices for further quantitative analysis of adenosine nucleotides. This systematic screening of ILMs also enhances the fundamental understanding of MALDI.
三磷酸腺苷（ATP）在生物系统中产生足够能量的代谢活动中发挥着重要作用。高 ATP/AMP 比值与糖尿病有关，会抑制 AMP 激活的蛋白激酶（AMPK）。基质辅助激光解吸/电离（MALDI）-质谱（MS）在确定样品中几种类型的腺苷磷酸的比值方面具有出色的潜力，可以快速了解代谢中的主要能量转移。尽管 MALDI 被视为 MS 分析的软电离技术，但过量的光子能量可能会破坏磷酸键，导致对 ATP 水平的错误解释。在这项工作中，离子液体基质（ILM）被用于减少 MALDI 过程中的碎裂并提高检测效率。本研究首次证明，2,5-二羟基苯甲酸吡啶（DHBP）是最有效的基质之一，可进一步对腺苷核苷酸进行定量分析。这种对 ILM 的系统筛选也增强了对 MALDI 的基本理解。
