Agahozo Marie Colombe, Timmermans Mieke A M, Sleddens Hein F B M, Foekens Renée, Trapman-Jansen Anita M A C, Schröder Carolien P, van Leeuwen-Stok Elise, Martens John W M, N M Dinjens Winand, van Deurzen Carolien H M
Department of Pathology, Erasmus MC Cancer Institute, 3015 GD Rotterdam, The Netherlands.
Department of Medical Oncology, Erasmus MC Cancer Institute, 3015GD Rotterdam, The Netherlands.
Cancers (Basel). 2020 Mar 9;12(3):631. doi: 10.3390/cancers12030631.
Loss of Y-chromosome (LOY) is associated with increased cancer mortality in males. The prevalence of LOY in male breast cancer (BC) is unknown. The aim of this study is to assess the presence and prognostic effect of LOY during male BC progression. We included male BC patients diagnosed between 1989 and 2009 (n = 796). A tissue microarray (TMA) was constructed to perform immunohistochemistry and fluorescent in situ hybridization (FISH), using an X and Y probe. We also performed this FISH on a selected number of patients using whole tissue slides to study LOY during progression from ductal carcinoma in situ (DCIS) to invasive BC. In total, LOY was present in 12.7% (n = 92) of cases, whereby LOY was associated with ER and PR negative tumors ( = 0.017 and = 0.01). LOY was not associated with the outcome. Using whole slides including invasive BC and adjacent DCIS (n = 22), we detected a concordant LOY status between both components in 17 patients. In conclusion, LOY is an early event in male breast carcinogenesis, which generally starts at the DCIS stage and is associated with ER and PR negative tumors.
Y染色体缺失（LOY）与男性癌症死亡率增加有关。LOY在男性乳腺癌（BC）中的患病率尚不清楚。本研究的目的是评估LOY在男性BC进展过程中的存在情况及其预后影响。我们纳入了1989年至2009年间诊断的男性BC患者（n = 796）。构建了组织微阵列（TMA），使用X和Y探针进行免疫组织化学和荧光原位杂交（FISH）。我们还对选定数量的患者使用全组织切片进行了这种FISH，以研究从原位导管癌（DCIS）进展为浸润性BC过程中的LOY情况。总体而言，12.7%（n = 92）的病例存在LOY，其中LOY与ER和PR阴性肿瘤相关（P = 0.017和P = 0.01）。LOY与预后无关。使用包括浸润性BC和相邻DCIS的全切片（n = 22），我们在17例患者中检测到两个成分之间的LOY状态一致。总之，LOY是男性乳腺癌发生过程中的早期事件，通常始于DCIS阶段，并且与ER和PR阴性肿瘤相关。
