LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira n 228, 4050-313 Porto, Portugal.
LAQV, REQUIMTE, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto, Rua de Jorge Viterbo Ferreira n 228, 4050-313 Porto, Portugal.
Molecules. 2020 Mar 15;25(6):1333. doi: 10.3390/molecules25061333.
This work proposes a simple and easy-to-use flow-through system for the implementation of dynamic extractions, aiming at the evaluation of bioaccessible zinc and the characterization of leaching kinetics in dry dog food samples. The kinetic profile of Zn extraction was determined by flame atomic absorption spectroscopy and the results were fitted in an exponential function (R > 0.960) compatible with a two first-order reactions model. Values of fast leachable Zn ranged from 83 ± 1 to 313 ± 5 mg of Zn per kg of sample, with associated rate constants ranging from 0.162 ± 0.004 to 0.290 ± 0.014 min. Similar results were observed compared to the static batch extraction. The percentage of bioaccessible Zn ranged from 49.0 to 70.0%, with an average value of 58.2% in relation to total Zn content. Principal component analysis regarding the variables fast leachable Zn, associated rate constant, total Zn, and market segment, has shown that 84.6% of variance is explained by two components, where the second component (24.0%) presented loadings only for the fast leachable Zn and associated rate constant. The proposed method is suitable for the fast evaluation (<1 h) of leaching kinetics and bioaccessibility in dry dog food.
本工作提出了一种简单易用的流动体系，用于实现动态提取，旨在评估干狗粮样品中的生物可利用锌和浸出动力学特性。通过火焰原子吸收光谱法测定 Zn 提取的动力学曲线，并将结果拟合到与两个一级反应模型兼容的指数函数（R > 0.960）中。快速可提取 Zn 的含量范围为 83 ± 1 至 313 ± 5 mg/kg 样品，对应的速率常数范围为 0.162 ± 0.004 至 0.290 ± 0.014 min。与静态批量提取相比，观察到类似的结果。生物可利用锌的百分比范围为 49.0%至 70.0%，与总 Zn 含量相比，平均值为 58.2%。关于快速可提取 Zn、相关速率常数、总 Zn 和市场细分变量的主成分分析表明，两个成分解释了 84.6%的方差，其中第二个成分（24.0%）仅对快速可提取 Zn 和相关速率常数有负载。该方法适用于干狗粮中浸出动力学和生物利用度的快速评估（<1 h）。
