State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
University of Science and Technology of China, Hefei, Anhui 230026, China.
Anal Chem. 2020 Apr 7;92(7):4755-4759. doi: 10.1021/acs.analchem.0c00042. Epub 2020 Mar 27.
A small, cheap, and low-power mesh-type piezoelectric ultrasonic transducer (MPUT) from a household USB humidifier has been developed as a sonochemiluminescence generator for the first time. The ultrasonication of an MPUT facilitates the generation of reactive oxygen species to trigger sonochemiluminescence. There is no light emission of luminol without sonication. In contrast, the luminescence becomes very intense by ultrasonication using the MPUT and can be readily observed by a smart phone, enabling the visual detection of luminol without adding any coreactants. Interestingly, ascorbic acid, a common chemiluminescence quencher in the literature, increases the sonochemiluminescence in this system. As a result, a sensitive sonochemiluminescence method has been developed for the visual detection of ascorbic acid with a linear range of 1-40 μM and a limit of detection (LOD) of 0.35 μM. Moreover, the visual detection of superoxide dismutase has been achieved on the basis of its quenching effect, which has a linear range of 0.05-2.0 μg/mL and a LOD of 0.018 μg/mL. Because of its advantages of low cost, small size, and low-power consumption, the USB MPUT holds great potential in sonochemiluminescence (SCL) for the development of portable and disposable analysis devices in point-of-care testing and field analysis as well as chemical education.
首次开发了一种来自家用 USB 加湿器的小型、廉价、低功耗的网格型压电超声换能器（MPUT）作为声致化学发光发生器。超声处理 MPUT 有助于产生活性氧物质来引发声致化学发光。没有声处理时，鲁米诺没有发光。相比之下，使用 MPUT 进行超声处理会使发光变得非常强烈，可以通过智能手机轻松观察到，从而可以在不添加任何辅助反应物的情况下对鲁米诺进行可视化检测。有趣的是，抗坏血酸是文献中常见的化学发光猝灭剂，在该体系中会增加声致化学发光。因此，开发了一种用于可视化检测抗坏血酸的灵敏声致化学发光方法，线性范围为 1-40 μM，检测限（LOD）为 0.35 μM。此外，基于其猝灭效应实现了对超氧化物歧化酶的可视化检测，其线性范围为 0.05-2.0 μg/mL，检测限（LOD）为 0.018 μg/mL。由于其低成本、小尺寸和低功耗的优势，USB MPUT 在声致化学发光（SCL）方面具有很大的潜力，可用于开发便携式和一次性分析设备，用于即时检测和现场分析以及化学教育。
