Hu Lianzhe, Hu Xilu, Huang Ting, Wang Min, Xu Guobao
Chongqing Key Laboratory of Green Synthesis and Applications, College of Chemistry, Chongqing Normal University, Chongqing 401331, China.
School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331, China.
ACS Omega. 2021 Feb 25;6(9):6379-6384. doi: 10.1021/acsomega.0c06301. eCollection 2021 Mar 9.
The phosphatase-like activity of Ce(IV) ions was applied for chemiluminescence (CL) analysis for the first time. Ce(IV) can catalyze the hydrolysis of CDP-star, which is a phosphatase substrate, to produce strong CL emission. The CL performance of the Ce(IV)/CDP-star system can be significantly improved by the addition of ionic liquids. In the presence of 1-butyl-3-methylimidazolium tetrafluoroborate, the selective and sensitive CL detection of Ce(IV) ions was achieved with a detection limit of 460 nM. The proposed CL system was also used for the detection of ascorbic acid and ClO. It is based on the phenomenon that Ce(IV) can catalyze the hydrolysis of CDP-star, while Ce(III) cannot. The introduction of reductive ascorbic acid into the mixture of Ce(IV)/CDP-star can turn off the CL signal, while the addition of oxidative ClO into the solution of Ce(III)/CDP-star can turn on the CL emission. Finally, Ce(IV)/CDP-star CL was successfully applied for evaluating the total antioxidant capacity in commercial fruit juice samples.
首次将铈（IV）离子的磷酸酶样活性应用于化学发光（CL）分析。铈（IV）可以催化磷酸酶底物CDP-star的水解，产生强烈的化学发光发射。通过添加离子液体可以显著提高铈（IV）/CDP-star体系的化学发光性能。在四氟硼酸1-丁基-3-甲基咪唑鎓存在下，实现了对铈（IV）离子的选择性和灵敏的化学发光检测，检测限为460 nM。所提出的化学发光体系还用于检测抗坏血酸和ClO。它基于铈（IV）可以催化CDP-star的水解，而铈（III）不能的现象。将还原性抗坏血酸引入铈（IV）/CDP-star混合物中可以关闭化学发光信号，而向铈（III）/CDP-star溶液中添加氧化性ClO可以开启化学发光发射。最后，铈（IV）/CDP-star化学发光成功应用于评估市售果汁样品中的总抗氧化能力。
