Faculty of Medicine, Department of Histology and Embryology, Mugla Sıtkı Kocman University, Mugla, Turkey.
Faculty of Medicine, Department of Histology and Embryology, Ege University, Izmir, Turkey.
Ultrastruct Pathol. 2020 Mar 3;44(2):193-202. doi: 10.1080/01913123.2020.1740366. Epub 2020 Mar 17.
Ovarian cancer is the seventh most common cancer worldwide in women. Many anticancer drugs are currently used clinically have been isolated from plant species or are based on such substances. Thymol (5-methyl-2-isopropylphenol) and carvacrol are oxygenated aromatic compounds from the monoterpene group. They are the main constituents of thyme essential oil and show antiproliferative, antioxidant, and antiseptic properties. The aim of this study is to compare the antiproliferative and apoptotic effects of thymol and carvacrol on SKOV-3 ovarian cancer cell line. The cancer cells were treated with different concentrations of thymol and carvacrol (100, 200, 400, 600 µM) at 24 h and 48 h durations. The cell viability was investigated by MTT assay and analysis of apoptosis with annexin V assay was determined. The study show that thymol and carvacrol significantly induced apoptosis in all groups as dose and time-dependent ( < .05). The data in the present study demonstrated that thymol and carvacrol have apoptotic and antiproliferative properties in a concentration-dependent manner toward ovarian cancer cells. SKOV-3 cancer cell line was much more sensitive to the toxic effect of thymol than carvacrol.
卵巢癌是全球女性中第七种最常见的癌症。目前临床上使用的许多抗癌药物都是从植物物种中分离出来的，或者是基于这些物质。百里酚（5-甲基-2-异丙基苯酚）和香芹酚是单萜类的含氧芳香族化合物。它们是百里香油的主要成分，具有抗增殖、抗氧化和防腐作用。本研究旨在比较百里酚和香芹酚对 SKOV-3 卵巢癌细胞系的增殖抑制和凋亡作用。用不同浓度的百里酚和香芹酚（100、200、400、600µM）处理癌细胞 24 小时和 48 小时。用 MTT 法检测细胞活力，用 Annexin V 法分析细胞凋亡。结果表明，百里酚和香芹酚在所有组中均呈剂量和时间依赖性显著诱导细胞凋亡（<0.05）。本研究的数据表明，百里酚和香芹酚对卵巢癌细胞具有浓度依赖性的促凋亡和抗增殖作用。SKOV-3 癌细胞系对百里酚的毒性作用比香芹酚更为敏感。
