Novato Tatiane Pinheiro Lopes, Araújo Laryssa Xavier, de Monteiro Caio Márcio Oliveira, Maturano Ralph, Senra Tatiane de Oliveira Souza, da Silva Matos Renata, Gomes Geovany Amorim, de Carvalho Mario Geraldo, Daemon Erik
Programa de Pós-graduação em Comportamento e Biologia Animal da Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário Bairro Martelos, 36036-330 Juiz de Fora, MG, Brazil.
Programa de Pós-graduação em Comportamento e Biologia Animal da Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário Bairro Martelos, 36036-330 Juiz de Fora, MG, Brazil.
Vet Parasitol. 2015 Sep 15;212(3-4):331-5. doi: 10.1016/j.vetpar.2015.08.021. Epub 2015 Aug 24.
This study aimed at assessing the combined effect of thymol, carvacrol and (E)-cinnamaldehyde on Amblyomma sculptum and Dermacentor nitens larvae. The effects resulting from treatments were evaluated by means of the modified larval packet test. In order to determine the LC50, components of essential oils, the monoterpenes thymol, carvacrol and phenylpropanoid (E)-cinnamaldehyde were individually tested at different concentrations. After determining the LC50, each essential oil component was separately evaluated and then combined with another substance at a 1:1 proportion at the LC50 concentration and at 1/2 and 1/4 of the LC50. For A. sculptum, the lowest LC50 value was obtained for (E)-cinnamaldehyde (1.40 mg/ml), followed by thymol (2.04 mg/ml) and carvacrol (3.49 mg/ml). The same order of effectiveness was observed for D. nitens, with values of 1.68, 2.17 and 3.33 mg/ml, respectively. In the evaluation of component associations of essential oils against A. sculptum larvae, only the combinations between carvacrol and thymol (LC50) and carvacrol and (E)-cinnamaldehyde (1/4 LC50) presented a moderate synergetic effect. In turn, for D. nitens larvae, the combinations between thymol and carvacrol (LC50 and 1/2 LC50) presented a synergetic effect, while the others presented an additive or antagonistic effect. Therefore, it can be concluded that the combination of thymol and carvacrol (LC50) has a moderate synergetic effect against A. sculptum larvae, while thymol, combined with carvacrol (LC50 and 1/2 LC50), has a synergetic effect against D. nitens larvae.
本研究旨在评估百里香酚、香芹酚和（E）-肉桂醛对刻点钝眼蜱和微小扇头蜱幼虫的联合作用。通过改良的幼虫包囊试验评估处理产生的效果。为了确定半数致死浓度（LC50），对精油成分单萜类百里香酚、香芹酚和苯丙烷类（E）-肉桂醛分别在不同浓度下进行测试。在确定LC50后，分别评估每种精油成分，然后将其与另一种物质按1:1比例在LC50浓度以及LC50的1/2和1/4浓度下混合。对于刻点钝眼蜱，（E）-肉桂醛获得最低的LC50值（1.40毫克/毫升），其次是百里香酚（2.04毫克/毫升）和香芹酚（3.49毫克/毫升）。微小扇头蜱也观察到相同的有效性顺序，其值分别为1.68、2.17和3.33毫克/毫升。在评估精油成分组合对刻点钝眼蜱幼虫的作用时，仅香芹酚与百里香酚（LC50）以及香芹酚与（E）-肉桂醛（1/4 LC50）的组合呈现出中等协同效应。反过来，对于微小扇头蜱幼虫，百里香酚与香芹酚（LC50和1/2 LC50）的组合呈现出协同效应，而其他组合呈现出相加或拮抗效应。因此，可以得出结论，百里香酚与香芹酚（LC50）的组合对刻点钝眼蜱幼虫具有中等协同效应，而百里香酚与香芹酚（LC50和1/2 LC50）的组合对微小扇头蜱幼虫具有协同效应。
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