Institute of Digestive Disease, Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR.
State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR.
Emerg Microbes Infect. 2020 Mar 17;9(1):631-638. doi: 10.1080/22221751.2020.1739564. eCollection 2020.
infection (CDI) is a common cause of nosocomial diarrhea and can sometimes lead to pseudo-membranous colitis and toxic megacolon. We previously reported that the PCR ribotype 002 was a common ribotype in Hong Kong that was associated with increased mortality. In this study, we assessed bacteriological characteristics and virulence of ribotype 002 compared to other common ribotypes, including ribotypes 012, 014 and 046. We observed significantly higher toxin A ( 0.05) and toxin B (< 0.05) production, sporulation ( 0.001) and germination rates (< 0.0001) in ribotype 002 than other common ribotypes. In a murine model of infection, ribotype 002 caused significantly more weight loss ( < 0.001) and histological damage ( < 0.001) than other common ribotypes. These findings may have contributed to the higher prevalence and mortality observed, and provided mechanistic insights that can help public surveillance and develop novel therapeutics to combat against this infection.
感染（CDI）是医院获得性腹泻的常见病因，有时可导致伪膜性结肠炎和中毒性巨结肠。我们之前曾报道过，PCR 核糖型 002 是香港常见的核糖型，与死亡率升高有关。在这项研究中，我们评估了核糖型 002 与其他常见核糖型（包括核糖型 012、014 和 046）相比的细菌学特征和毒力。我们观察到，核糖型 002 的毒素 A（0.05）和毒素 B（<0.05）的产生、孢子形成（0.001）和发芽率（<0.0001）显著高于其他常见核糖型。在感染的小鼠模型中，核糖型 002 导致的体重减轻（<0.001）和组织学损伤（<0.001）明显多于其他常见核糖型。这些发现可能导致了更高的流行率和死亡率，并提供了有助于公共监测和开发新型治疗方法以对抗这种感染的机制见解。
