Universidade Federal de Santa Catarina (UFSC), Centro de Ciências da Saúde, Departamento de Análises Clínicas, Laboratório de Estudos de Protozoários Emergentes e Oportunistas, CEP: 88040-970, Florianópolis, Santa Catarina, Brazil.
Universidade Federal de Santa Catarina (UFSC), Centro de Ciências da Saúde, Departamento de Análises Clínicas, Laboratório de Biologia Molecular, Sorologia e Micobactérias, CEP 88040-900, Florianópolis, Santa Catarina, Brazil.
Parasitology. 2020 Jun;147(7):782-790. doi: 10.1017/S0031182020000487. Epub 2020 Mar 18.
Free-living amoebae (FLA) are widely dispersed in the environment, can cause opportunistic and non-opportunistic infections in humans and other animals. The aim of the present study was characterize FLA obtained from air-conditioners of a public hospital in the city of Florianópolis, SC, Brazil. Fifty-four dust samples were collected of air conditioners, and were inoculated on 1.5% non-nutrient agar, overlaid with layers of Escherichia coli. Subsequently the isolates were axenised in PYG growth medium. The morphological and molecular characterization of the isolates was performed, as well as the tolerance (physiological) assays were used to evaluate the pathogenic potential. The results revealed the presence of FLA in 42 (77.8%) of the collected samples. Of these, 39 (92.9%) axenic isolates of FLA were obtained for morphological and genotypic studies. All the isolates characterized belong to the genus Acanthamoeba. Nineteen (48.7%) isolates belong to the genotype T4, 16 (41.0%) to the T5 genotype and 4 (10.3%) to genotype T11. Seven (18.0%) isolates were considered potentially pathogenic in tolerance assays. These findings require attention, considering the isolation environment and immunocompromised characteristics of many hospitalized patients.
自由生活阿米巴（FLA）广泛分布于环境中，能引起人类和其他动物的机会性和非机会性感染。本研究的目的是对巴西圣卡塔琳娜州弗洛里亚诺波利斯市一家公立医院空调中获得的 FLA 进行特征描述。采集了 54 份空调灰尘样本，并接种于 1.5%非营养琼脂上，用一层大肠杆菌覆盖。随后将分离物在 PYG 生长培养基中进行无共生培养。对分离物进行形态和分子特征描述，并进行耐受力（生理）检测以评估其潜在致病性。结果显示，在所采集的 42 份（77.8%）样本中存在 FLA。其中，39 个（92.9%）FLA 分离物经形态学和基因型研究被鉴定为无菌培养物。所有鉴定的分离物均属于棘阿米巴属。19 个（48.7%）分离物属于 T4 基因型，16 个（41.0%）属于 T5 基因型，4 个（10.3%）属于 T11 基因型。在耐受力检测中，有 7 个（18.0%）分离物被认为具有潜在致病性。考虑到许多住院患者的隔离环境和免疫功能低下的特点，这些发现需要引起重视。
