Department of Earth Sciences, Resources and Environmental Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo, 169-8555, Japan.
Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan.
Biochem Biophys Res Commun. 2020 May 14;525(4):1081-1086. doi: 10.1016/j.bbrc.2020.03.013. Epub 2020 Mar 14.
Cell morphology is related to proliferation and differentiation. We previously reported that cell attachment area of rat mesenchymal stem cells (MSCs) is negatively correlated with their osteogenic differentiation level on osteoconductive hydroxyapatite (HAp) with various microstructures. In this study, the correlation between the cell attachment area and osteogenic differentiation level was investigated on substrates without osteoconductive property using tissue culture polystyrene (TCPS), and 3 mol% yttria-stabilized zirconia (3Y-TZP) with or without surface periodic microstructures. It was found that the osteogenic differentiation level after 3 weeks of culture increased with a decrease in cell attachment area after 3 h of culture. The square of the correlation coefficient between cell attachment area and osteocalcin secretion content was 0.845 among the three types of substrates. Thus, the negative correlation between cell attachment area and differentiation level is confirmed even when cultured on substrates without osteoconductive property. These findings suggest that the correlation between the cell attachment area of rat MSCs and osteogenic differentiation level could also apply to various types of substrate, regardless of osteoconductive property.
细胞形态与增殖和分化有关。我们之前曾报道，在具有不同微观结构的骨诱导性羟磷灰石（HAp）上，大鼠间充质干细胞（MSCs）的细胞黏附面积与成骨分化水平呈负相关。在这项研究中，使用组织培养聚苯乙烯（TCPS）和具有或没有表面周期性微观结构的 3mol%氧化钇稳定氧化锆（3Y-TZP），在没有骨诱导性的基质上研究了细胞黏附面积与成骨分化水平之间的相关性。结果发现，培养 3 周后的成骨分化水平随培养 3 小时后的细胞黏附面积的减少而增加。在这三种基质中，细胞黏附面积与骨钙素分泌含量之间的相关系数的平方为 0.845。因此，即使在没有骨诱导性的基质上培养，也可以确认细胞黏附面积与分化水平之间的负相关性。这些发现表明，大鼠 MSCs 的细胞黏附面积与成骨分化水平之间的相关性也适用于各种类型的基质，而与骨诱导性无关。
