表皮生长因子受体变异体 III 靶向适配体偶联金纳米颗粒治疗脑胶质瘤。
Aptamer-Conjugated Gold Nanoparticles Targeting Epidermal Growth Factor Receptor Variant III for the Treatment of Glioblastoma.
机构信息
Key Laboratory of Mental Health of the Ministry of Education, Guangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired Intelligence, Guangdong Province Key Laboratory of Psychiatric Disorders, Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, People's Republic of China.
The Fifth Affiliated Hospital, Southern Medical University, Guangzhou 510900, People's Republic of China.
出版信息
Int J Nanomedicine. 2020 Feb 28;15:1363-1372. doi: 10.2147/IJN.S238206. eCollection 2020.
PURPOSE
In this study, we constructed novel brain-targeting complexes (U2-AuNP) by conjugating aptamer U2 to the gold nanoparticle (AuNPs) surface as a promising option for GBM therapy.
MATERIALS AND METHODS
The properties of the U2-AuNP complexes were thoroughly characterized. Then, we detected the in vitro effects of U2-AuNP in U87-EGFRvIII cell lines and the in vivo antitumor effects of U2-AuNP in GBM-bearing mice. Furthermore, we explored the inhibition mechanism of U2-AuNP in U87-EGFRvIII cell lines.
RESULTS
We found that U2-AuNP inhibits the proliferation and invasion of U87-EGFRvIII cell lines and prolongs the survival time of GBM-bearing mice. We found that U2-AuNP can inhibit the EGFR-related pathway and prevent DNA damage repair in GBM cells.
CONCLUSION
These results reveal the promising potential of U2-AuNP as a drug candidate for targeted therapy in GBM.
目的
本研究通过将适体 U2 与金纳米粒子（AuNPs）表面缀合，构建了新型的脑靶向复合物（U2-AuNP），为 GBM 治疗提供了一种有前途的选择。
材料和方法
对 U2-AuNP 复合物的性质进行了全面表征。然后，我们检测了 U2-AuNP 在 U87-EGFRvIII 细胞系中的体外效应，以及 U2-AuNP 在荷 GBM 小鼠体内的抗肿瘤作用。此外，我们还探索了 U2-AuNP 在 U87-EGFRvIII 细胞系中的抑制机制。
结果
我们发现 U2-AuNP 可抑制 U87-EGFRvIII 细胞系的增殖和侵袭，并延长荷 GBM 小鼠的生存时间。我们发现 U2-AuNP 可抑制 EGFR 相关通路并阻止 GBM 细胞中的 DNA 损伤修复。
结论
这些结果表明 U2-AuNP 作为一种针对 GBM 的靶向治疗药物候选物具有很大的潜力。
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