Hou Ying, Yuan Peipei, Fu Yang, Zhang Qi, Wei Yaxin, Gao Liyuan, Liu Li, Wang Xiaolan, Zheng Xiaoke, Feng Weisheng
Henan University of Chinese Medicine, Zhengzhou 450046, China.
Guizhou HanFang Pharmaceutical Co., Ltd., Guizhou 550081, China.
Evid Based Complement Alternat Med. 2020 Feb 27;2020:6723204. doi: 10.1155/2020/6723204. eCollection 2020.
Duzhong Butiansu (DZBTS) prescription contains many traditional Chinese medicines and has been shown to have a curative effect on male fertility. However, the efficacy and mechanism of DZBTS in the treatment of male infertility induced by heat stress have not been reported. The aim of the present study is to elucidate the effect and mechanism of DZBTS on spermatogenic function of a heat stress model in rats.
Male Wistar rats (280-320 g) were given different doses of DZBTS (0.4853 g/kg/d or 0.9707 g/kg/d), Shengjing capsule (0.56 g/kg/d), or double distilled water for 15 days. A 43°C hot water bath for 30 minutes was used to stimulate the testis of rats. Sperm count, sperm motility, the organ index of kidney and gonadal organs, serum sex hormone levels, and serum oxidising reaction index were measured. Haematoxylin and eosin (HE) staining was used to observe the morphology of the testis and kidney. The expression of Hsp70 in testes was observed by immunofluorescence. The changes in heat stress, reproductive-related protein, and mRNA were measured by western blot assay and RT-qPCR.
Heat stress downregulated the levels of sex hormone ( < 0.05 or < 0.05 or < 0.05 or < 0.05 or < 0.05 or < 0.05 or < 0.05 or.
Our results for the first time have found that DZBTS can improve spermatogenesis disorder in a heat stress model in rats, which may be mainly by regulating AR, sperm regulatory protein CREB1, and the HSF/Hsp70 signaling pathway to decrease oxidative stress.
杜仲补天素（DZBTS）方剂包含多种中药，已被证明对男性生育能力有治疗作用。然而，DZBTS治疗热应激诱导的男性不育症的疗效和机制尚未见报道。本研究的目的是阐明DZBTS对大鼠热应激模型生精功能的影响及机制。
将雄性Wistar大鼠（280 - 320 g）给予不同剂量的DZBTS（0.4853 g/kg/d或0.9707 g/kg/d）、生精胶囊（0.56 g/kg/d）或双蒸水，持续15天。用43°C热水浴30分钟刺激大鼠睾丸。检测精子计数、精子活力、肾脏和性腺器官的器官指数、血清性激素水平以及血清氧化反应指标。采用苏木精-伊红（HE）染色观察睾丸和肾脏的形态。通过免疫荧光观察睾丸中Hsp70的表达。采用蛋白质免疫印迹法和逆转录-定量聚合酶链反应（RT-qPCR）检测热应激、生殖相关蛋白和mRNA的变化。
热应激下调了性激素水平（< 0.05或< 0.05或< 0.05或< 0.05或< 0.05或< 0.05或< 0.05或。
我们的结果首次发现DZBTS可改善大鼠热应激模型中的生精障碍，这可能主要是通过调节雄激素受体（AR）、精子调节蛋白CREB1以及热休克因子/热休克蛋白70（HSF/Hsp70）信号通路来降低氧化应激。
