Li Jun-Tao, Qu Xiao-Wei, Zhang Shu-Wu, Li Zheng-Sheng, Zhang Pei-Hai
Department of Andrology, The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450008, China.
Department of Urology, Zhengzhou People's Hospital, Zhengzhou, Henan 450003, China.
Zhonghua Nan Ke Xue. 2016 Dec;22(12):1110-1115.
To study the possible pathogenesis of infertility caused by dibutyl phthalate (DBP) and investigate the effects of Yishen Shengjing Capsules (YSC, kidney-tonifying and essence-producing capsules) on DBP-induced reproductive function injury and its possible action mechanisms in male Wistar rats.
Models of DBP-induced reproductive function injury were made in 80 male Wistar rats and another 20 were used as blank controls. After modeling, the model rats were randomly divided into a model control, a high-dose YSC, a medium-dose YSC, and a low-dose YSC group. Four weeks after intervention, all the animals were sacrified for observation of the histomorphologic changes in the testis under the light microscope, measurement of sperm concentration, motility and abnormality, and determination of the levels of serum testosterone (T), luteinizing hormone (LH) and follicle-stimulating hormone (FSH) by radioimmunoassay.
Compared with the blank controls, the model rats showed obvious pathological changes in testicular histomorphology, significantly decreased sperm concentration and motility, increased sperm abnormality, reduced contents of serum T and LH, and elevated the level of serum FSH (P<0.01). After 4 weeks of medication, the animals of the high-, medium-, and low-dose YSC groups, in comparison with the model controls, exhibited different degrees of recovery from testicular histomorphological damage, remarkably increased sperm concentration and motility, decreased sperm abnormality, elevated levels of serum T and LH, and reduced content of serum FSH (P<0.01). There were statistically significant differences in all the parameters above between the high-dose YSC and medium- and low-dose YSC groups (P<0.01).
DBP reduces sperm motility and concentration, increases sperm abnormality, causes damage to the morphological structure of the rat testis, decreases the contents of serum T and LH, and elevates the level of the serum FSH. Yishen Shengjing Capsules can improve DBP-induced productive function injury, increase sperm motility and concentration, decrease sperm abnormality, elevate the level of serum T and LH, reduce the content of serum FSH, improve the morphological structure of the testis, and thus promote the reproductive function of the male rat.
研究邻苯二甲酸二丁酯（DBP）致不育的可能发病机制，探讨益肾生精胶囊（YSC，补肾生精胶囊）对DBP所致雄性Wistar大鼠生殖功能损伤的影响及其可能作用机制。
将80只雄性Wistar大鼠制成DBP致生殖功能损伤模型，另20只作为空白对照。造模后，将模型大鼠随机分为模型对照、YSC高剂量、YSC中剂量和YSC低剂量组。干预4周后，处死所有动物，光镜下观察睾丸组织形态学变化，检测精子浓度、活力和畸形率，并采用放射免疫法测定血清睾酮（T）、黄体生成素（LH）和卵泡刺激素（FSH）水平。
与空白对照组相比，模型大鼠睾丸组织形态学出现明显病理变化，精子浓度和活力显著降低，精子畸形率增加，血清T和LH含量降低，血清FSH水平升高（P<0.01）。用药4周后，YSC高、中、低剂量组动物与模型对照组相比，睾丸组织形态学损伤均有不同程度恢复，精子浓度和活力显著增加，精子畸形率降低，血清T和LH水平升高，血清FSH含量降低（P<0.01）。YSC高剂量组与中、低剂量组上述各项指标比较，差异均有统计学意义（P<0.01）。
DBP降低精子活力和浓度，增加精子畸形率，损伤大鼠睾丸形态结构，降低血清T和LH含量，升高血清FSH水平。益肾生精胶囊可改善DBP所致生殖功能损伤，增加精子活力和浓度，降低精子畸形率，升高血清T和LH水平，降低血清FSH含量，改善睾丸形态结构，从而促进雄性大鼠生殖功能。
