Butcher Chelsea L, Rubin Berish Y, Anderson Sylvia L, Nandula Vijay K, Owen Micheal D K, Gardner Randolph G, Lewis J D
Louis Calder Center, Biological Field Station Fordham University 31 Whippoorwill Road Armonk New York 10504 USA.
Center for Urban Ecology Fordham University 441 East Fordham Road Bronx New York 10458 USA.
Appl Plant Sci. 2020 Mar 4;8(3):e11330. doi: 10.1002/aps3.11330. eCollection 2020 Mar.
Pollen dispersal plays a critical role in gene flow of seed plants. Most often, pollen dispersal is measured using paternity assignment. However, this approach can be time-consuming because it typically entails genotyping all pollen donors, receptors, and offspring at several molecular markers.
We developed a faster, simpler protocol to track paternity, using pollen receptors and grouped pollen donors that possess rare alleles. We tested this approach using wind-pollinated and insect-pollinated . After screening potential markers for rare alleles, we grew both species in experimental arrays under field conditions.
All tested . seeds and 97% of . fruits could be assigned to the grouped pollen donors using each of two markers. From these results, we could infer paternity of untested offspring and assess pollen dispersal patterns in each array.
By combining rare alleles and grouped pollen donors, we could assess pollen dispersal for both species and across all arrays after genotyping a small number of pollen donors and a representative subset of offspring. While directly applicable to and , this approach could be used in other species to assess pollen dispersal under field conditions.
花粉传播在种子植物的基因流动中起着关键作用。大多数情况下，花粉传播是通过父本鉴定来衡量的。然而，这种方法可能很耗时，因为它通常需要对所有花粉供体、受体和后代进行多个分子标记的基因分型。
我们开发了一种更快、更简单的追踪父本的方法，使用花粉受体和拥有稀有等位基因的分组花粉供体。我们通过风媒传粉植物和虫媒传粉植物对这种方法进行了测试。在筛选出具有稀有等位基因的潜在标记后，我们在田间条件下的实验阵列中种植了这两个物种。
使用两个标记中的任何一个，所有测试的[植物名称1]种子和97%的[植物名称2]果实都可以归为分组花粉供体。从这些结果中，我们可以推断未测试后代的父本情况，并评估每个阵列中的花粉传播模式。
通过结合稀有等位基因和分组花粉供体，在对少量花粉供体和后代的代表性子集进行基因分型后，我们可以评估这两个物种以及所有阵列中的花粉传播情况。虽然该方法直接适用于[植物名称1]和[植物名称2]，但也可用于其他物种以评估田间条件下的花粉传播。
