Yamada G, Tsuji T
First Department of Internal Medicine, Okayama University Medical School, Japan.
Acta Pathol Jpn. 1988 Jun;38(6):759-68. doi: 10.1111/j.1440-1827.1988.tb02347.x.
Seventy-two patients with HBsAg-positive chronic hepatitis were studied for serum antibody to delta antigen (anti-HD) by radioimmunoassay. Delta antigen (HDAg) in liver of the 72 patients was also studied immunohistochemically using peroxidase-labeled anti-HD. In one of the 72 patients, high titers of anti-HD and intrahepatic HDAg were detected. In this patient, HDAg was localized mainly in nuclei and occasionally in the cytoplasm of hepatocytes. By immunoelectron microscopy, HDAg was observed diffusely in some hepatocytic nuclei, but their nucleoli, perichromatin and heterochromatin were devoid of HDAg. Occasionally, inclusion body-like ring-shaped or irregularly shaped aggregates of HDAg were observed in nuclei. At higher magnification, these aggregates consisted of an HDAg-positive amorphous substance and microtubular structures. In their vicinity, scattered HDAg-positive granules were also present. By conventional electron microscopy, similar inclusion bodies composed of a clustered amorphous substance and microtubular structures 10-20 nm in diameter, together with 20-30 nm irregular granules were observed in hepatocyte nuclei, but no core of Dane particles were found. These novel intranuclear structures were not observed in other patients with chronic hepatitis B. It is suggested that they may represent the characteristic ultrastructure associated with delta infection.
采用放射免疫分析法对72例HBsAg阳性慢性肝炎患者的血清δ抗原抗体（抗-HD）进行了研究。同时，使用过氧化物酶标记的抗-HD对这72例患者肝脏中的δ抗原（HDAg）进行了免疫组织化学研究。在这72例患者中，有1例检测到高滴度的抗-HD和肝内HDAg。在该患者中，HDAg主要定位于肝细胞核内，偶尔也见于肝细胞的细胞质中。通过免疫电子显微镜观察，在一些肝细胞核内可弥漫性观察到HDAg，但核仁、染色质周边和异染色质中未发现HDAg。偶尔，在核内可见包涵体样的环形或不规则形HDAg聚集物。在高倍放大下，这些聚集物由HDAg阳性的无定形物质和微管结构组成。在其附近，也存在散在的HDAg阳性颗粒。通过传统电子显微镜观察，在肝细胞核内可见由聚集的无定形物质和直径为10 - 20nm的微管结构组成的类似包涵体，以及20 - 30nm的不规则颗粒，但未发现Dane颗粒核心。在其他慢性乙型肝炎患者中未观察到这些新的核内结构。提示它们可能代表与δ感染相关的特征性超微结构。
