Department of Civil Engineering, National Institute of Technology, Nagaoka College, 888 Nishikatakaimachi, Nagaoka, Niigata, 940-8532, Japan.
Department of Applied Chemistry and Bioscience, Chitose Institute of Science and Technology, 758-65 Bibi, Chitose, Hokkaido, 066-8655, Japan.
Biotechnol Lett. 2020 Jun;42(6):979-985. doi: 10.1007/s10529-020-02865-6. Epub 2020 Mar 17.
To synthesize hydrazine (NH) from ammonium and hydroxylamine (NHOH) using an anaerobic ammonium oxidation (anammox) bacterium, Candidatus Kuenenia stuttgartiensis.
K. stuttgartiensis cells were anoxically cultivated with the addition of ammonium (2 mM) and NHOH (1-100 mM) at pH 6-10.5, and 4-65 °C to examine the favorable cultivation conditions for NH production. The influence of NHOH concentration was more prominent than that of pH and temperature, and NHOH concentration higher than 1 mM deteriorated NH yields significantly. The following conditions were found to be favorable for NH production using K. stuttgartiensis cells: pH 9, 38 °C, and < 1 mM NHOH. In a continuous-feed system operated at these conditions, K. stuttgartiensis cells produced NH with a maximum concentration of 0.65 mM, which is the highest NH concentration previously reported in biological processes.
Optimal cultivation conditions for K. stuttgartiensis for NH production were successfully determined, and the present study is the first to document potential biological NH production using anammox bacteria.
利用厌氧氨氧化菌 Candidatus Kuenenia stuttgartiensis 从铵盐和羟胺（NHOH）合成肼（NH）。
在添加铵盐（2 mM）和 NHOH（1-100 mM）的条件下，将 K. stuttgartiensis 细胞在无氧条件下于 pH 6-10.5 和 4-65°C 下培养，以考察 NH 生成的适宜培养条件。NHOH 浓度的影响比 pH 和温度的影响更为显著，NHOH 浓度高于 1 mM 会显著降低 NH 的产率。使用 K. stuttgartiensis 细胞生产 NH 的适宜条件为：pH 9、38°C 和<1 mM NHOH。在这些条件下运行的连续进料系统中，K. stuttgartiensis 细胞以 0.65 mM 的最大浓度产生 NH，这是生物过程中先前报道的最高 NH 浓度。
成功确定了 K. stuttgartiensis 用于 NH 生产的最佳培养条件，本研究首次记录了利用厌氧氨氧化菌进行潜在的生物 NH 生产。
